Duffy antigen receptor for chemokines attenuates breast cancer growth and metastasis:an experiment with nude mice
文献类型：期刊论文
| 作者 | Wang Jie; Ou Zhouluo; Hou Yifeng; Luo Jianmin; Chen Yi ; Zhou Jin; Shen Zhenzhou; King Jian; Shao zhimin
|
| 刊名 | National Medical Journal of China
 |
| 出版日期 | 2005 |
| 卷号 | 85期号:29页码:2033-2037 |
| 关键词 | Breast cancer Metastasis Neovascularity |
| ISSN号 | 0376-2491 |
| 其他题名 | Duffy抗原趋化因子受体抑制人乳腺癌裸鼠移植瘤的生长和肺转移 |
| 文献子类 | Article |
| 英文摘要 | Objective To study the effects of Duffy antigen receptor for chemokines (DARC) on the tumorigenesis and metastasis of breast cancer. Methods Human breast cancer cells of the line MDA-MB^35HM with a great potentiality of metastasis were cultured and then divided into 3 groups; MDA-MB-435HM-DARC cell group, to be transfected with pcDNA3/DARC FyB containing human DARC cDNA; MDA-MB^35HM-vect cell group, to be transfected with blank plasmid pcDNA3; and MDA-MB-435HM cell group without transfection. Female BALB/c nude mice were implanted with MDA-MB-435HM cells into the nipple. The size of the implanted cancer was measured once a week. The mice were killed 4,6, and 8 weeks after the implantation respectively and their lungs were taken out to observe the number of metastatic tumor. Immunohistochemical staining was performed to calculate the microvascular density ( MVD). Western blotting was used to detect the matrix metalloproteinase ( MMP) -9 protein expression of the lung tissues. ELISA was used to detect the concentrations of the 2 DARC ligands: CNCL8 and CCL2 in the supernatant of culture fluid of the 3 kinds of cells and the metastatic tumors. Results The expression levels of DARC mRNA and protein of the MDA-MB435HM cells were 38% those of the MDA-MB435 cells. The expression levels of CXCL8 and CCL2 of the MDA-MB435HM-DARC cells were significantly lower than those of the MDA-MB-435HM-vect cells and MDA-MB435HM cells (both P <0. 05). All mice developed tumor with a tumorigenesis rate of 100% . However, the tumor occurred 17 days after implantation in those mice implanted with MDA-MB-435HM-DARC cells, significantly later then in those implanted with MDA-MB-435HM-vect cells and MDA-MB-435HM cells (9 and 10 days after implantation) the size of tumor in the former group being significantly smaller than those of the 2 latter groups ( both P < 0. 01) . The number of metastatic foci in the lung of the MDA-MB435HM-DARC group was significantly less than those of the other 2 groups (both P <0. 01). The level of CCI2 of the implanted tumor in the MDA-MB435HM-DARC group was significantly lower than those of the other 2 groups ( both P < 0. 01 ) . The MVD of the MDA-MB-435HM-DARC was significantly less than those of the other 2 groups ( both P < 0. 01) and most of the vessels of the MDA-MB435HM-DARC group were not newly formed vessels with a diameter < 8 red blood. |
| 资助项目 | 国家自然科学基金资助项目[00000000] ; 国家杰出青年科学基金资助项目[00000000] ; 上海市科委重点项目基金资助项目[00000000] ; 上海市自然科学基金资助项目[00000000] |
| WOS研究方向 | Oncology (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2150544 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268283]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | xBreast Cancer Center, Cancer Hospital, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. |
| 推荐引用方式 GB/T 7714 | Wang Jie,Ou Zhouluo,Hou Yifeng,et al. Duffy antigen receptor for chemokines attenuates breast cancer growth and metastasis:an experiment with nude mice[J]. National Medical Journal of China,2005,85(29):2033-2037. |
| APA | Wang Jie.,Ou Zhouluo.,Hou Yifeng.,Luo Jianmin.,Chen Yi.,...&Shao zhimin.(2005).Duffy antigen receptor for chemokines attenuates breast cancer growth and metastasis:an experiment with nude mice.National Medical Journal of China,85(29),2033-2037. |
| MLA | Wang Jie,et al."Duffy antigen receptor for chemokines attenuates breast cancer growth and metastasis:an experiment with nude mice".National Medical Journal of China 85.29(2005):2033-2037. |
入库方式： OAI收割
来源：上海药物研究所
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