Silence of Paxillin and P130Cas by RNAi Inhibited Growth of Gastric Cancer Cells
文献类型：期刊论文
| 作者 | Li Ying2; Yao Libo2; Liu Xinping2; Wang Lifeng2; Han Yueheng2; Lin Shuxin1; Shu Qiang3 |
| 刊名 | Chinese Journal of Biochemistry and Molecular Biology
 |
| 出版日期 | 2005 |
| 卷号 | 21期号:3页码:408-414 |
| ISSN号 | 1007-7626 |
| 关键词 | RNAi paxillin p130Cas RNAi anoikis paxillin p130Cas gastric cancer cell |
| 其他题名 | RNAi引起的Paxillin和p130Cas下调抑制胃癌细胞失巢性生长 |
| 文献子类 | Article |
| 英文摘要 | Focal adhension kinase (FKA) is a key component of the signal transduction pathways triggered by integrins and activation of FAK lead to acts on potential substrates paxiUin and pl30Cas, which causes increases cell migration and potentially regulates cell proliferation. To investigate the effects of the two signal molecules on anoikis (apoptosis that occurs in cells that are detached from matrix). RNA interference technolgy was applied to silence the genes of the two proteins in gastric cancer cell line BGC823 and observed the the effects on ceU anchorage independent growth. According to the mechanism of RNAi technology, two target sequences of paxillin and pl30Cas gene were designed and subsequently, two vectors (pWHl-/)130cas, pWHl-paxilin) were constructed.Following trasient transfected into BGC823 cell line cultured both in adhence and suspension, the mRNA and protein expression level of the two genes were significantly decreased by RT-PCR and Western blot assay. Furthermore, the inverted phase contrast microscope demonstrated that the attached BGC823 cell shrank and detached from the matrix and that clustering phenomenon of suspended cells were inhibited following the two molecules were being silenced, respectively. MTT assay also showed that the survival rate of the detached BGC823 pWHl-paxillin and GGC823 pWHl-pl30Cas were markedly decrease to 32.19% and 28,52% as compared with control group (P < 0.01) .This was mainly due to the enhanced Gl phase as demonstrated by FCM assay.The above results suggested that pl30Cas and paxillin have played an important role in the gastric cancer cell resistant to anoilks. |
| 资助项目 | 国家自然科学基金[00000000] ; 军队"十五"科研基金重点课题[00000000] |
| WOS研究方向 | Cell Biology ; Genetics & Heredity (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1996435 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268298]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Department of Pathophysiology, Fourth Military Medical University, Xian, Shaanxi 710033, China.; 2.Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xian, Shaanxi 710033, China.; 3.Institute of Pharmacology, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Li Ying,Yao Libo,Liu Xinping,et al. Silence of Paxillin and P130Cas by RNAi Inhibited Growth of Gastric Cancer Cells[J]. Chinese Journal of Biochemistry and Molecular Biology,2005,21(3):408-414. |
| APA | Li Ying.,Yao Libo.,Liu Xinping.,Wang Lifeng.,Han Yueheng.,...&Shu Qiang.(2005).Silence of Paxillin and P130Cas by RNAi Inhibited Growth of Gastric Cancer Cells.Chinese Journal of Biochemistry and Molecular Biology,21(3),408-414. |
| MLA | Li Ying,et al."Silence of Paxillin and P130Cas by RNAi Inhibited Growth of Gastric Cancer Cells".Chinese Journal of Biochemistry and Molecular Biology 21.3(2005):408-414. |
入库方式： OAI收割
来源：上海药物研究所
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