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The preliminary studies on the cross action between endothelin-1 and angiotensin Ⅱ in kidney tissue of rats with diabetic nephropathy
文献类型：期刊论文
| 作者 | Cheng Hong; Tian Xuefei; Dong Hongrui; Ding Shuangshuang; Qiu Changbin; Chen Yipu |
| 刊名 | National Medical Journal of China
 |
| 出版日期 | 2004 |
| 卷号 | 84期号:3页码:248-252 |
| 关键词 | endothelin-1 angiotensin II diabetic nephropathies |
| ISSN号 | 0376-2491 |
| 其他题名 | 糖尿病肾病大鼠肾脏内皮素-1与血管紧张素Ⅱ交叉作用的初步探讨 |
| 文献子类 | Article |
| 英文摘要 | Objective To investigate the interrelation between endothelin-1 ( ET-1 ) and angiotensin E (Ang II) in kidney tissue of rats with diabetic nephropathy. Methods Wistar rats were performed a removal operation of right kidney. Two weeks later the uninephrectomized rats were given intravenous injection of streptozotocin ( STZ,35 mg/kg ). The diabetic rats were randomly divided into thefollowing four groups; DM + bos group ( bosentan 100 mg/kg/d by gavage) ; DM + ena group ( enalapril 10 mg/kg/d by gavage); DM + bos + ena group (the same doses of both bosentan and enalapril by gavage) ; DM + veh group (only buffer by gavage) . Besides, uninephrectomized rats without STZ injection were assigned as control group. Each group consisted of 6 rats. Twenty weeks later, they were sacrificed and left kidney of each rat was harvested respectively. The mRNA expression of angiotensinogen (Ao) .angiotensin type 1 receptor (AT1R) ,preproendothelin-l and endothelin A receptor (ETaR) , and the protein expression of Ang II , AT1R, ET-1 and ETaR in kidney tissue were semi-quantitatively detected with reverse transcription- polymerase chain reaction and immunohistochemical staining respectively. Results In the diabetic group without treatment (DM + veh group) , the expression of Ao( A E ) , AT1R and ET-AR was significantly up-regulated (1. 25, 1. 94 and 2. 56-folds in mRNA respectively, 2. 52, 3. 84 and 3. 30-folds in protein respectively, P<0. 01 or P <0. 05) compared with control group. In three treatment groups, i. e. DM + bos group, DM + ena group and DM + bos + ena group, the up-regulated expression of Ao (A H ) , AT1R and ET-AR was significantly attenuated ( -38.2%～-54. 8% in mRNA and -55. 3%～-69.7% in protein, P < 0. 05 or P < 0. 01) compared with DM group. The inhibitory rates among these three treatment groups had no significant difference (P > 0. 05 ). In this study, the expression of preproendothelin-1 raRNA and ET-1 protein was not significantly changed among all groups (P >0. 05). Conclusion Either endothelin receptor antagonist or ACE inhibitor can significantly inhibit the expression of AngH , AT1R and ETaR in kidney tissue of diabetic rats, which suggest that there are close relationship and cross action between ET-1 and Ang E. |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1605557 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268362]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Center of Nephrology, China-Japan Friendship Hospital, Beijing 100029, China. |
| 推荐引用方式 GB/T 7714 | Cheng Hong,Tian Xuefei,Dong Hongrui,et al. The preliminary studies on the cross action between endothelin-1 and angiotensin Ⅱ in kidney tissue of rats with diabetic nephropathy[J]. National Medical Journal of China,2004,84(3):248-252. |
| APA | Cheng Hong,Tian Xuefei,Dong Hongrui,Ding Shuangshuang,Qiu Changbin,&Chen Yipu.(2004).The preliminary studies on the cross action between endothelin-1 and angiotensin Ⅱ in kidney tissue of rats with diabetic nephropathy.National Medical Journal of China,84(3),248-252. |
| MLA | Cheng Hong,et al."The preliminary studies on the cross action between endothelin-1 and angiotensin Ⅱ in kidney tissue of rats with diabetic nephropathy".National Medical Journal of China 84.3(2004):248-252. |
入库方式： OAI收割
来源：上海药物研究所
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