The preliminary study of intervention role of enalapril and bosentan in rats with diabetic nephropathy
文献类型：期刊论文
| 作者 | Cheng Hong; Tian Xuefei; Dong Hongrui |
| 刊名 | Chinese Journal of Diabetes
 |
| 出版日期 | 2004 |
| 卷号 | 12期号:6页码:437-441 |
| 关键词 | Diabetic nephropathy Collagen Transforming growth factor-beta Plasminogen activator inhibitor-1 Tissue inhibitor of matrix metalloproteinase-1 |
| ISSN号 | 1006-6187 |
| 其他题名 | 依那普利及波生坦对糖尿病肾病大鼠干预作用的初步研究 |
| 文献子类 | Article |
| 英文摘要 | Objective To investigate the pathogenesis and intervention measures of diabetic nephropa-thy. Methods Uninephrectomized Wistar rats were given intravenously streptozotocin (STZ) . The diabetic rats were randomly divided into four groups by gavage: Veh group (only buffer); Bos group (bosentan 100 mg/kg/d); Ena group (enalaprii 10 mg/kg/d); Bos+Ena group (the same doses of both bosentan and enalaprii). Besides, uninephrectomized rats without STZ injection were assigned as control group. Each group consisted of 6 rats. By twenty weeks, the body weight, blood pressure, blood glucose and proteinuria were measured in each group. Then, the rats were sacrificed and the remnant kidney of each rat was harvested and weighed respectively. The mRNA and the protein expression of collagen KQ) .colla-gen ]y (C1V) (transforming growth factor-beta_1, (TGF-beta_1 ) ,plasminogen activator inhibitor-1 (PAI-1), tissue in-hibitor of matrix metalloproteinase-1 (TIMP-1) in kidney tissue were semi-quantitatively detected with re-verse transcription- polymerase chain reaction (RT-PCR) and immunohistochemical staining respectively. Results In Veh group the mean arterial pressure (MAP), blood glucose level, urinary protein excretion, and the ratio of kidney weight/body weight were significantly increased compared with control group (P< 0. 05), and the expression of C_I,C_(IV), TGF-beta_1 , PAI-1 and TIMP-1 mRNA and protein in kidney tissue was also significantly up-regulated compared with control group (P<0. 05). In three treatment groups the increased above- mentioned parameters except blood glucose were significantly attenuated compared with Veh group (P<0. 05) , but there were no significant differences in the parameters among these three groups (P >0. 05). Conclusions Either endothelin receptor antagonist bosentan or ACE inhibitor enalaprii has defi-nite therapeutic effects on diabetic nephropathy. These effects might come from the decreased C_I and C_(IV) pro-duction in the diabetic kidney tissue resulted from the down-regulation of TGF-beta_1, PAI-1 and TIMP-1 expression by bosentan or/and enalaprii. |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1673736 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268363]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Center of Nephrology, China-Japan Friendship Hospital, Beijing 100029, China. |
| 推荐引用方式 GB/T 7714 | Cheng Hong,Tian Xuefei,Dong Hongrui. The preliminary study of intervention role of enalapril and bosentan in rats with diabetic nephropathy[J]. Chinese Journal of Diabetes,2004,12(6):437-441. |
| APA | Cheng Hong,Tian Xuefei,&Dong Hongrui.(2004).The preliminary study of intervention role of enalapril and bosentan in rats with diabetic nephropathy.Chinese Journal of Diabetes,12(6),437-441. |
| MLA | Cheng Hong,et al."The preliminary study of intervention role of enalapril and bosentan in rats with diabetic nephropathy".Chinese Journal of Diabetes 12.6(2004):437-441. |
入库方式： OAI收割
来源：上海药物研究所
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