A comparison study between bosentan and enalapril treatment in diabetic nephropathy
文献类型：期刊论文
| 作者 | Cheng Hong; Tian Xuefei; Dong Hongrui; Ding Shuangshuang; Qiu Changbin; Chen Yipu |
| 刊名 | Chinese Journal of Nephrology
 |
| 出版日期 | 2004 |
| 卷号 | 20期号:3页码:154-158 |
| 关键词 | Diabetic nephropathy Collagen Transforming growth factor Connective tissue growth factor-beta1 Bone morphogenetic protein-7 |
| ISSN号 | 1001-7097 |
| 其他题名 | 波生坦及依那普利对糖尿病肾病模型干预的对比研究 |
| 文献子类 | Article |
| 英文摘要 | Objective To compare different effects of treatment with bosentan or enalapril in diabetic nephropathy, and investigate their pharmacological mechanisms. Methods Uninephrectomized Wistar rats were given intravenously streptozotocin(STZ) . The diabetic rats were randomly divided the following: DN +veh group (only buffer by gavage), DN + bos group (bosentan 100 mg·kg~(-1)·d~(-1) by gavage), DN + ena group (enalapril 10 mg·kg~(-1)·d~(-1) by gavage) .An additional set of uninephrectomized rats were given no STZ were used as control group (CTR). Each group consisted of 6 rats. By twenty weeks, the body weight, blood pressure, blood glucose and proteinuria level were measured in each rat. Then, the rats were sacrificed and the remnant kidney of each rat was harvested and weighed. The mRNA and the protein expression of collagen I (Coll), collagenW (Col W), transforming growth factor-beta1(TGF-beta1), connective tissue growth factor (CTGF), bone morphogenetic protein-7(BMP-7) in kidney tissue were semi-quantitatively determined with reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining respectively. Results Compared with CTR, the mean arterial pressure (MAP), serum glucose level, urinary protein excretion, and the ratio of kidney weight/body weight were significantly increased(P < 0.05 or 0.01), and the relative area of glomerular mesangium and basement membrane and the relative area of interstitial fibrosis were also significantly enlarged in the DN+veh group( P < 0.01). Compared with CTR, the expression of Col I,Col IV,TGF-beta1 and CTGF mRNA and protein was significantly up-regulated, but the expression of BMP-7 mRNA and protein was significantly down-regulated in their kidney tissue of DN + veh group (P < 0. 05 or 0. 01) . After intervention with bosentan or enalapril, the upregulation of the above-mentioned parameters, except blood glucose, were all significantly inhibited(P < 0.05 or 0.01), but the down-regulated BMP-7 expression was significantly enhanced (P < 0.01). There was no significant difference of intervention effects between bosentan and enalapril (P > 0. 05) . Conclusions In this study, pharmacological effects of bosentan or enalapril on diabetic nephropathy rats are quite similar. These effects may result from the down-regulation of TGF-beta1 and CTGF expression and up-regulation of BMP-7 expression in kidney tissue. |
| WOS研究方向 | Surgery (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1851256 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268364]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Center of Nephrology, China-Japan Friendship Hospital, Beijing 100029, China. |
| 推荐引用方式 GB/T 7714 | Cheng Hong,Tian Xuefei,Dong Hongrui,et al. A comparison study between bosentan and enalapril treatment in diabetic nephropathy[J]. Chinese Journal of Nephrology,2004,20(3):154-158. |
| APA | Cheng Hong,Tian Xuefei,Dong Hongrui,Ding Shuangshuang,Qiu Changbin,&Chen Yipu.(2004).A comparison study between bosentan and enalapril treatment in diabetic nephropathy.Chinese Journal of Nephrology,20(3),154-158. |
| MLA | Cheng Hong,et al."A comparison study between bosentan and enalapril treatment in diabetic nephropathy".Chinese Journal of Nephrology 20.3(2004):154-158. |
入库方式： OAI收割
来源：上海药物研究所
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