Orthotopic xenograft of Wilms' tumor in nude mice:Biological characteristics of the models
文献类型：期刊论文
| 作者 | He Xiaowei; Yuan Shengtao; Gao Jiechun |
| 刊名 | Chinese Journal of Pediatric Surgery
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:3页码:258-260 |
| 关键词 | Nephroblastoma Disease models,animal Neoplasm transplantation |
| ISSN号 | 0253-3006 |
| 其他题名 | 人肾母细胞瘤裸小鼠肾原位移植模型的建立和生物学特性的研究 |
| 文献子类 | Article |
| 英文摘要 | Objective To establish orthotopic xenograft models of human Wilms' tumor in nude mice. Methods Histologically intacted human Wilms' tumor fragments from one female baby ( stage II ) in 14 months of age were transplanted into subrenal capsular space of nude mice. Subsequent generations of transplantations were carried out in similar fashion. The first, second, third and fourth generations of tumor transplants numbered 2, 5, 5 and 18 respectively. The models were removed and weighed after 8 weeks. The first, second and third generations of mice were sacrificed after 8 weeks. Every 3 nude mice out of fourth generation were sacrificed weekly from week 3 to 8 to study tumor growth dynamics. The cell cycle distribution, apoptosis and ploidy of the original tumor and the transplants were studied with flow cy-tometry. Tumors were examined for morphology and immuno-stained for intermediate filaments, CK18, vimentin and desmin. PCR amplification of three microsatellites D14S68, D18S69 and, D20S199 in genome were performed to clarify its human origin. Results Twenty-seven out of 30 orthotopic xenografts of human Wilm's tumor were detected in nude mice after four generations. Its growth curve was exponential. DNA index and cell cycle/apoptosis distributions were similar in each generation. All transplanted tumors retained the original histological and immunohistochernical staining characters. Furthermore, mi-crosatellite DNA sequences in genome of transplanted tumors were identical with that of original human Wilms' tumor. Conclusions The orthotopic xenograft model of human Wilms' tumor is a close reproduction of the original tumor. It serves as a valuable tool in the biological and therapeutic study of human Wilms' tumor. |
| WOS研究方向 | Pediatrics (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1301262 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268425]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | Department of Pediatric Surgery,Affiliated Children's Hospital,Fudan University, Shanghai 200032, China. |
| 推荐引用方式 GB/T 7714 | He Xiaowei,Yuan Shengtao,Gao Jiechun. Orthotopic xenograft of Wilms' tumor in nude mice:Biological characteristics of the models[J]. Chinese Journal of Pediatric Surgery,2003,24(3):258-260. |
| APA | He Xiaowei,Yuan Shengtao,&Gao Jiechun.(2003).Orthotopic xenograft of Wilms' tumor in nude mice:Biological characteristics of the models.Chinese Journal of Pediatric Surgery,24(3),258-260. |
| MLA | He Xiaowei,et al."Orthotopic xenograft of Wilms' tumor in nude mice:Biological characteristics of the models".Chinese Journal of Pediatric Surgery 24.3(2003):258-260. |
入库方式： OAI收割
来源：上海药物研究所
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