Isolation,Culture and Identification of Bone Marrow-derived Mesenchymal Stem Cells from Arbas Cashmere Goat
文献类型：期刊论文
| 作者 | Ma Suriguga1; Peng Hang2; Liu Jiajia3; Zhang Yiting4; Gui Hua1; Liu Pengxia1 |
| 刊名 | Biotechnology Bulletin
 |
| 出版日期 | 2016 |
| 卷号 | 32期号:2页码:203-210 |
| 关键词 | Arbas cashmere goat mesenchymal stem cells culture differentiate biological characteristics |
| ISSN号 | 1002-5464 |
| 其他题名 | 阿尔巴斯绒山羊骨髓间充质干细胞体外分离培养及鉴定 |
| 文献子类 | Article |
| 英文摘要 | Here we isolated and cultured Arbas cashmere goat bone marrow-derived mesenchymal stem cells(gBM-MSCs)in vitro, identified the biological characteristics of gBM-MSCs, and analyzed the dynamic expressions of differentiation genes while induced in vitro. The primary cultured gBM-MSCs were spindle cell-based, showing radial colony arrangement, and stably and continuously passed down for over 15 generations. The detections by immunofluorescence and flow cytometry showed that gBM-MSCs were positive for mesenchymal specific markers, such as CD13, CD29, CD44, CD106, CD90 and CD166, but negative for hematopoietic stem cell surface markers CD45 and CD34. The induction in vitro confirmed that gBM-MSCs owned the multiple differentiation potential, and differentiated into adipocytes, osteoblasts and chondrocyte. Real-time PCR further indicated that the expressions of differentiation genes increased throughout the culture period. Our results confirmed that the isolated and cultured cells from the bone marrow of Arbas cashmere goat possessed the biological characteristics of gBM-MSCs. Our findings promoted the basic research of MSCs in livestock and laid a theoretical foundation for the further application of gBM-MSCs in transgenic breeding and tissue engineering. |
| 资助项目 | 国家自然科学基金项目[00000000] ; 内蒙古杰出青年自然科学基金项目[00000000] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5645535 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269258]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.College of Life Sciences,Inner Mongolia University, Hohhot, Inner Mongolia 010021, China.; 2.Bone and Joints Research Center,Frontier Institute of Science and Technology,Xian Jiaotong University, Xian, 710061.; 3.Institute of Microbiology,Chinese Academy of Sciences, Beijing 100101, China.; 4.Center for Drug Safety Evaluation and Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Ma Suriguga,Peng Hang,Liu Jiajia,et al. Isolation,Culture and Identification of Bone Marrow-derived Mesenchymal Stem Cells from Arbas Cashmere Goat[J]. Biotechnology Bulletin,2016,32(2):203-210. |
| APA | Ma Suriguga,Peng Hang,Liu Jiajia,Zhang Yiting,Gui Hua,&Liu Pengxia.(2016).Isolation,Culture and Identification of Bone Marrow-derived Mesenchymal Stem Cells from Arbas Cashmere Goat.Biotechnology Bulletin,32(2),203-210. |
| MLA | Ma Suriguga,et al."Isolation,Culture and Identification of Bone Marrow-derived Mesenchymal Stem Cells from Arbas Cashmere Goat".Biotechnology Bulletin 32.2(2016):203-210. |
入库方式： OAI收割
来源：上海药物研究所
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