Rabbit Chondrocytes Apoptosis Models Induced by Different Concentrations of SNP
文献类型：期刊论文
| 作者 | Chen Qixin; Han Guanying; Guo Bin
|
| 刊名 | Chinese General Practice
 |
| 出版日期 | 2015 |
| 卷号 | 18期号:24页码:2939-2945 |
| 关键词 | Nitroprusside Chondrocytes Apoptosis |
| ISSN号 | 1007-9572 |
| 其他题名 | 不同浓度硝普钠诱导兔软骨细胞凋亡模型的比较研究 |
| 文献子类 | Article |
| 英文摘要 | Objective To compare the chondrocytes apoptosis models induced by different concentrations of sodium nitroprusside (SNP),in order to provide theoretical support for the further investigation of the regulating effect of drugs on chondrocytes apoptosis. Methods Eight 4-week-old healthy New Zealand male rabbits (weight,200-250 g) were selected from September to December in 2014.Rabbit chondrocytes were cultured and researched in vitro.Different concentrations of SNP (0.062 5,0.125,0.25,0.5,1,2 mmol/L) were used to induce rabbit chondrocytes.At 12,24 and 48 h,MTT method and TUNEL method were employed to evaluate the chondrocytes apoptosis models induced by different concentrations of SNP.The OD value and apoptosis rate of rabbit chondrocytes were recorded. Results At different time points,chondrocytes apoptosis models induced by different concentrations of SNP were significantly different (P<0.05) in OD value.At 12,24 and 48 h,chondrocytes apoptosis models induced by 0.062 5 mmol/L and 0.125 mmol/L of SNP were higher (P<0.05) than the chondrocytes apoptosis model induced by 0 mmol/L of SNP in OD value;at 12,24 and 48 h,chondrocytes apoptosis models induced by 0.25,0.5,1 and 2 mmol/L of SNP were lower (P<0.05) than the chondrocytes apoptosis model induced by 0 mmol/L of SNP in OD value;at 12,24 and 48 h,chondrocytes apoptosis models induced by 0.25,0.5,1 and 2 mmol/L of SNP were significantly different (P<0.05) in OD value.At different time points,chondrocytes apoptosis models induced by different concentrations of SNP were significantly different (P<0.05) in apoptosis rate.At 12,24 and 48 h,chondrocytes apoptosis models induced by 0.25,0.5,1 and 2 mmol/L of SNP were higher (P<0.05) than the chondrocytes apoptosis model induced by 0 mmol/L of SNP in apoptosis rate;chondrocytes apoptosis models induced by different concentrations of SNP were significantly different (P<0.05) in apoptosis rate. Conclusion Chondrocytes apoptosis models of different severity degrees can be build by SNP with the concentration ranging from 0.25 mmol/L to 2 mmol/L,which provides theoretical support for the regulating effect of drugs on chondrocytes apoptosis. |
| 资助项目 | 国家自然科学基金资助项目[00000000] ; 国家"十二五"重大新药创制基金[00000000] ; 辽宁省自然科学基金资助项目[00000000] ; 辽宁医学院校长基金-奥鸿博泽基金[00000000] ; 辽宁医学院校长基金-全民口腔研究生科研创新基金[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5500844 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269273]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | College of Pharmacy,Liaoning Medical University, Jinzhou, Liaoning 121001, China. |
| 推荐引用方式 GB/T 7714 | Chen Qixin,Han Guanying,Guo Bin. Rabbit Chondrocytes Apoptosis Models Induced by Different Concentrations of SNP[J]. Chinese General Practice,2015,18(24):2939-2945. |
| APA | Chen Qixin,Han Guanying,&Guo Bin.(2015).Rabbit Chondrocytes Apoptosis Models Induced by Different Concentrations of SNP.Chinese General Practice,18(24),2939-2945. |
| MLA | Chen Qixin,et al."Rabbit Chondrocytes Apoptosis Models Induced by Different Concentrations of SNP".Chinese General Practice 18.24(2015):2939-2945. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。