Discussions on the Inaccuracy Problems and Preventing Strategies Associated with the Use of Liquid Chromatography-Tandem Mass Spectrometry in Quantitative Assay of Biosamples
文献类型：期刊论文
| 作者 | Wei Minji1; Li Li2; Zhang Yuhu2; Yang Yong3; Zhong Dafang3
|
| 刊名 | Chinese Pharmaceutical Journal
 |
| 出版日期 | 2015 |
| 卷号 | 50期号:11页码:925-930 |
| 关键词 | LC-MS /MS biosample inaccuracy bioanalytical risk pitfall matrix effect in-source conversion |
| ISSN号 | 1001-2494 |
| 其他题名 | 液相色谱-串联质谱在生物样品定量测定中存在的不准确因素分析和对策 |
| 文献子类 | Review |
| 英文摘要 | Liquid chromatography coupled with tandem mass spectrometry (LC-MS /MS)is a widely used technique in the quantitative analysis of small molecules,part of peptides and proteins in biological matrix. When compared with LC,the LC-MS /MS posesses many advantages,such as high sample throughput,high sensitivity and resolutions,simple method development and simultaneous quantification of multiple components. At the stage of method development,the main practices for analysts are to validate analytical methods according to the regulated requirements. However,due to the quantitative mechanisms of LC-MS /MS,even though the used method is fully validated,analytical inaccuracy problem,which is the so called bioanalytical risk (pitfalls),may still exist,if we do not pay attentions to some details. In the present paper,the factors which may cause inaccuracy in quantitative analysis are summarized, especially those could not be found during routine method validation. Those factors include back transformation of unstable drug metabolites to analyte in the handling of biosamples,matrix effects,in-source conversion,and ion interference caused by ions with similar or same mass-to-charge ratio,etc. In addition to QA/QC oversight and strict method validation,the preventing strategies may contain the follows: selecting biosample processing method with high proficiency in eliminating matrix interferences,enhancing chromatographic separation,selection of suitable precursor-product ions and validation with incurred samples,etc. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5447055 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269278]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Institute of Clinical Pharmacology,First Hospital, Peking University, Beijing 100191, China.; 2.Center for Drug Evaluation,China Food and Drug Administration, Beijing 100038, China.; 3.Center of Drug Metabolism,Institute of Materia Medica,Chinese Academy of Science, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Wei Minji,Li Li,Zhang Yuhu,et al. Discussions on the Inaccuracy Problems and Preventing Strategies Associated with the Use of Liquid Chromatography-Tandem Mass Spectrometry in Quantitative Assay of Biosamples[J]. Chinese Pharmaceutical Journal,2015,50(11):925-930. |
| APA | Wei Minji,Li Li,Zhang Yuhu,Yang Yong,&Zhong Dafang.(2015).Discussions on the Inaccuracy Problems and Preventing Strategies Associated with the Use of Liquid Chromatography-Tandem Mass Spectrometry in Quantitative Assay of Biosamples.Chinese Pharmaceutical Journal,50(11),925-930. |
| MLA | Wei Minji,et al."Discussions on the Inaccuracy Problems and Preventing Strategies Associated with the Use of Liquid Chromatography-Tandem Mass Spectrometry in Quantitative Assay of Biosamples".Chinese Pharmaceutical Journal 50.11(2015):925-930. |
入库方式： OAI收割
来源：上海药物研究所
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