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Establishment and Application of High -throughput Screening Model for SHP2 Inhibitors

文献类型：期刊论文

作者Gao Wei1; Shao Wei2; Su Gang2; Li Jia3; Xie Xiaodong2
刊名Science & Technology Review
出版日期2014
卷号32期号:4-5页码:91-94
关键词SHP2 SHP2 inhibitor high throughput screening
ISSN号1000-7857
其他题名蛋白酪氨酸磷酸酶SHP2抑制剂高通量筛选模的建立和应用用
文献子类Article
英文摘要The aims of this study is to set up a high throughput screening model for SHP2 inhibitors and screen the potential inhibitors. By the E. coil expression system GST-SHP2 fusion protein is cloned and over expressed. High-purity SHP2 protein is purified though GST-Sepharose column. After establishing the high through screening system with the colorimetric assay of SHP2, a library of 48000 pure compounds is screened using the 384 micro- plate. Among them 75 compounds have inhibitory effects over 50%. Ultimately, three inhibitors are identified as SHP2 inhibitors with high activity. The high throughput screening is a highly sensitive, inexpensive, and operationally simple assay method in identifying SHP2 inhibitors.
资助项目国家自然科学基金项目[00000000]
WOS研究方向Biotechnology & Applied Microbiology (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:5087719
源URL[http://119.78.100.183/handle/2S10ELR8/269347]  
专题中国科学院上海药物研究所
作者单位1.Department of General Thoracic Surgery, Lanzhou General Hospital of Lanzhou Military Command, Lanzhou, Gansu 730050, China.;
2.Key Lab of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science of Lanzhou University, Lanzhou, Gansu 730020, China.;
3.National Center for Drug Screening, Shanghai lnstitute of Materia Medica, Chinese Academy of Science, Shanghai 201203, China.
推荐引用方式
GB/T 7714		 Gao Wei,Shao Wei,Su Gang,et al. Establishment and Application of High -throughput Screening Model for SHP2 Inhibitors[J]. Science & Technology Review,2014,32(4-5):91-94.
APA Gao Wei,Shao Wei,Su Gang,Li Jia,&Xie Xiaodong.(2014).Establishment and Application of High -throughput Screening Model for SHP2 Inhibitors.Science & Technology Review,32(4-5),91-94.
MLA Gao Wei,et al."Establishment and Application of High -throughput Screening Model for SHP2 Inhibitors".Science & Technology Review 32.4-5(2014):91-94.

入库方式： OAI收割

来源：上海药物研究所

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