Structure and RNA recognition of ribosome assembly factor Utp30
文献类型：期刊论文
| 作者 | Hu, Jianfei1,2,3,4; Zhu, Xing1; Ye, Keqiong1,4 |
| 刊名 | RNA
 |
| 出版日期 | 2017-12 |
| 卷号 | 23期号:12页码:1936-1945 |
| 关键词 | ribosome assembly 90S preribosome crystal structure RNA-binding protein |
| ISSN号 | 1355-8382 |
| DOI | 10.1261/rna.062695.117 |
| 文献子类 | Article |
| 英文摘要 | The 90S preribosomes are gigantic early assembly intermediates of small ribosomal subunits. Cryo-EM structures of 90S were recently determined, but many of its components have not been accurately modeled. Here we determine the crystal structure of yeast Utp30, a ribosomal L1 domain-containing protein in 90S, at 2.65 angstrom resolution, revealing a classic two-domain fold. The structure of Utp30 fits well into the cryo-EM density of 90S, confirming its previously assigned location. Utp30 binds to the rearranged helix 41 of 18S rRNA and helix 4 of 5' external transcribed spacer in 90S. Comparison of RNA-binding modes of different L1 domains illustrates that they consistently recognize a short RNA duplex with the concaved surface of domain I, but are versatile in RNA recognition outside the core interface. Cic1 is a paralog of Utp30 associating with large subunit preribosomes. Utp30 and Cic1 share similar RNA-binding modes, suggesting that their distinct functions may be executed by a single protein in other organisms. Deletion of Utp30 does not affect the composition of 90S. The nonessential role of Utp30 could be ascribed to its peripheral localization and redundant interactions in 90S. |
| WOS关键词 | EUKARYOTIC RIBOSOME ; SACCHAROMYCES-CEREVISIAE ; NUCLEAR EXPORT ; BIOGENESIS ; YEAST ; SUBUNITS |
| 资助项目 | National Natural Science Foundation of China[91540201] ; National Natural Science Foundation of China[31430024] ; National Natural Science Foundation of China[31325007] ; Chinese Academy of Sciences[XDB08010203] ; National Key R&D Program of China[2017YFA0504600] ; CAS[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000415372800017 |
| 出版者 | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272389]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ye, Keqiong |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Key Lab RNA Biol, Beijing 100101, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Jianfei,Zhu, Xing,Ye, Keqiong. Structure and RNA recognition of ribosome assembly factor Utp30[J]. RNA,2017,23(12):1936-1945. |
| APA | Hu, Jianfei,Zhu, Xing,&Ye, Keqiong.(2017).Structure and RNA recognition of ribosome assembly factor Utp30.RNA,23(12),1936-1945. |
| MLA | Hu, Jianfei,et al."Structure and RNA recognition of ribosome assembly factor Utp30".RNA 23.12(2017):1936-1945. |
入库方式： OAI收割
来源：上海药物研究所
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