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Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency

文献类型：期刊论文

作者Abulaiti, Xianmixinuer1; Zhang, Han2,3,4,5; Wang, Aifang2,4; Li, Na2,3,4; Li, Yang1; Wang, Chenchen1,2,4; Du, Xiaojuan1; Li, Lingsong1,2,3,4,5
刊名STEM CELL REPORTS
出版日期2017-11-14
卷号9期号:5页码:1630-1641
ISSN号2213-6711
DOI10.1016/j.stemcr.2017.09.001
文献子类Article
英文摘要OCT4 is required to maintain the pluripotency of embryonic stem cells (ESCs); yet, overdose-expression of OCT4 induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this differentiation switch is not fully understood. Here, we found that substitution of threonine(343) by alanine (T343A), but not aspartic acid (T343D), caused a significant loss of OCT4-phosphorylation signal in ESCs. Loss of such OCT4-phosphorylation compromises its interaction with SOX2 but promotes interaction with SOX17. We therefore propose that threonine(343)-based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency. This OCT4-phosphorylation-based mechanism may provide insight into the regulation of lineage specification during early embryonic development.
WOS关键词POU DOMAIN ; SELF-RENEWAL ; PREIMPLANTATION EMBRYO ; LINEAGE SPECIFIERS ; ES CELLS ; EXPRESSION ; NANOG ; PHOSPHOPROTEOME ; DIFFERENTIATION ; CIRCUITRY
资助项目863 Program from the Ministry of Science and Technology of China[2015AA020303] ; Zhang Jiang National Innovation and Shanghai Bureau of Science and Technology[ZJ2014-ZD-002] ; National Natural Science Foundation of China[31671217] ; National Natural Science Foundation of China[31000653] ; National Natural Science Foundation of China[31571517]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000415139400025
出版者CELL PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/272404]  
专题中国科学院上海药物研究所
通讯作者Wang, Chenchen; Li, Lingsong
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China;
2.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China;
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Abulaiti, Xianmixinuer,Zhang, Han,Wang, Aifang,et al. Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency[J]. STEM CELL REPORTS,2017,9(5):1630-1641.
APA Abulaiti, Xianmixinuer.,Zhang, Han.,Wang, Aifang.,Li, Na.,Li, Yang.,...&Li, Lingsong.(2017).Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency.STEM CELL REPORTS,9(5),1630-1641.
MLA Abulaiti, Xianmixinuer,et al."Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency".STEM CELL REPORTS 9.5(2017):1630-1641.

入库方式： OAI收割

来源：上海药物研究所

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