Low molecular weight xanthan gum suppresses oxidative stress-induced apoptosis in rabbit chondrocytes
文献类型：期刊论文
| 作者 | Chen, Qixin2,3; Shao, Xintian3; Ling, Peixue2,3; Liu, Fei2,3; Shao, Huarong3; Ma, Aibin2,3; Wu, Jixu2,3; Zhang, Wei1; Liu, Fuyan2,3; Han, Guanying1,3,4 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2017-08-01 |
| 卷号 | 169页码:255-263 |
| 关键词 | Xanthan gum Low molecular weight Oxidative stress Chondrocytes Apoptosis |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2017.04.018 |
| 文献子类 | Article |
| 英文摘要 | We have previously reported the application of low molecular weight XG(LM-XG), with molecular weights ranging from 1 x 10(6) Da to 1.5 x 10(6) Da for treating osteoarthritis. In this study, we investigated the anti-apoptotic activity of LM-XG under oxidative stress conditions, activated by hydrogen peroxide (H2O2) -treated chondrocytes in vitro. Chondrocytes were pretreated with various doses of LM-XG (0,10, 100, 500, or 1000 mu g/mL) or 1000 mu g/mL sodium hyaluronate for 12 h, and then exposed to 0.5 mmol/L H2O2 for another 12 h. After treatment, chondrocyte viability was evaluated using a cell counting kit-8; DNA fragmentation was detected using Hoechst33258 staining; the percentage of DNA fragmentation was evaluated using the diphenylamine DNA assay kit; the apoptosis rate was evaluated using flow cytometry; chondrocyte ultra -microscopic morphology was observed using transmission electron microscopy; intracellular reactive oxygen species levels were observed and quantified using 2,7-dichlorofuorescin diacetate, mitochondrial permeability transition analysis was performed using MitoTracker Red CMXRos and 4',6-diamidino-2-phenylindole staining; and finally, caspase-3 activity was detected by western blot. The results showed that, compared with H2O2 -treated chondrocytes, LM-XG improved cell viability, decreased the percentage of DNA fragmentation, reduced the apoptosis rate, decreased the levels of intracellular reactive oxygen species and mitochondrial permeability transition, reverted the morphological damage, and downregulated cleaved caspase-3 levels. These results demonstrate that LM-XG has anti-apoptotic activity in H2O2 -treated chondrocytes. (C) 2017 Elsevier Ltd. All rights reserved. |
| WOS关键词 | NITROPRUSSIDE-INDUCED APOPTOSIS ; PROTECTS CHONDROCYTES ; HYDROGEN-PEROXIDE ; IN-VITRO ; CARTILAGE DEGRADATION ; OSTEOARTHRITIS MODEL ; HYALURONIC-ACID ; CELLS ; MITOCHONDRIA ; DAMAGE |
| 资助项目 | National Major Scientific and Technological Special Project for "Significant New Drugs Development" during the Twelfth Five-year Plan Period[2012ZX093016-002] ; National Natural Science Foundation of China[81302681] ; Development Plan of the Outstanding Young Scholars of Liaoning Province[LJQ2015064] ; Natural Science Foundation Master Plan of Liaoning Province[201602282] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000402343000030 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272551]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ling, Peixue; Han, Guanying; Wang, Fengshan |
| 作者单位 | 1.Jinzhou Med Univ, Affiliated Hosp 1, Jinzhou 121001, Liaoning Provin, Peoples R China; 2.Shandong Univ, Sch Pharmaceut Sci, Jinan 250101, Shangdong Provi, Peoples R China; 3.Shandong Acad Pharmaceut Sci, Key Lab Biopharmaceut, Engn Lab Polysaccharide Drugs, Natl Local Joint Engn Lab Polysaccharide Drugs, Jinan 250101, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Qixin,Shao, Xintian,Ling, Peixue,et al. Low molecular weight xanthan gum suppresses oxidative stress-induced apoptosis in rabbit chondrocytes[J]. CARBOHYDRATE POLYMERS,2017,169:255-263. |
| APA | Chen, Qixin.,Shao, Xintian.,Ling, Peixue.,Liu, Fei.,Shao, Huarong.,...&Wang, Fengshan.(2017).Low molecular weight xanthan gum suppresses oxidative stress-induced apoptosis in rabbit chondrocytes.CARBOHYDRATE POLYMERS,169,255-263. |
| MLA | Chen, Qixin,et al."Low molecular weight xanthan gum suppresses oxidative stress-induced apoptosis in rabbit chondrocytes".CARBOHYDRATE POLYMERS 169(2017):255-263. |
入库方式： OAI收割
来源：上海药物研究所
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