Separation, purification and structure analysis of an extracellular polysaccharides from Antarctic bacterium Pseudoalteromonas sp s-15-13
文献类型：期刊论文
| 作者 | Li Jiang2; Song Guo-Qiang3; Chen Kao-Shan1,2; Li Guang-You2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2008-06-10 |
| 卷号 | 29期号:6页码:1149-1152 |
| 关键词 | extracelluar polysaccharide antarctic bacterium Pseudoalteronwnas sp S-15-13 separation and purification |
| ISSN号 | 0251-0790 |
| 文献子类 | Article |
| 英文摘要 | An immunoactive extracelluar polysaccharide was isolated from Pseudoalteromonas sp. S-15-13, a bacterium screened from Antarctic sea-ice. In order to accurately define the structure of the Antarctic bacterial EPSs and to relate these findings to the function of these molecules in the natural environment, purification and structural analysis of S-15-13 EPSs was carried out. Exopolysaccharide fractions were extracted and were purified by DEAE-Sephadex A-50 ion-exchange and Sephadex G-100 gel chromatography to give EPS-II. The EPS-II was eluted as a single peak in HPLC analysis, indicating that the homogeneity and purity of EPS-II were suited to the structural analysis. The molecular weight of the EPS-II was determined as 62000 by the HPLC method. Sugar composition analysis, methylation analysis and one-dimensional and two-dimensional NMR spectroscopies reveal that the main structure of EPS-II was a 1 2 linking mannan with fewer 1 2, 6-linking branch, a new extracelluar polysaccharide from Antarctic bacterium. |
| WOS研究方向 | Chemistry |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3338122 |
| WOS记录号 | WOS:000257176300017 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272893]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li Jiang |
| 作者单位 | 1.Shandong Univ, Coll Life Sci, Jinan 250100, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioactive Subst, Qingdao 266061, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Materia Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li Jiang,Song Guo-Qiang,Chen Kao-Shan,et al. Separation, purification and structure analysis of an extracellular polysaccharides from Antarctic bacterium Pseudoalteromonas sp s-15-13[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2008,29(6):1149-1152. |
| APA | Li Jiang,Song Guo-Qiang,Chen Kao-Shan,&Li Guang-You.(2008).Separation, purification and structure analysis of an extracellular polysaccharides from Antarctic bacterium Pseudoalteromonas sp s-15-13.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,29(6),1149-1152. |
| MLA | Li Jiang,et al."Separation, purification and structure analysis of an extracellular polysaccharides from Antarctic bacterium Pseudoalteromonas sp s-15-13".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 29.6(2008):1149-1152. |
入库方式： OAI收割
来源：上海药物研究所
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