Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies
文献类型：期刊论文
| 作者 | Ikeda, Rie1; Nishimura, Miho1,2; Sun, Yen; Wada, Mitsuhiro1; Nakashima, Kenichiro1 |
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2008-06 |
| 卷号 | 22期号:6页码:630-636 |
| 关键词 | Cordyceps sinensis Cordyceps militaris nucleosides authentication quality control HPLC-UV |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.980 |
| 文献子类 | Article |
| 英文摘要 | A simple HPLC-UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C-18 column by isocratic elution with acetonitrile-water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine in Cordyceps were 0.28-14.15, 0.006-6.36, 0.01-0.14, 0.68-14.79 and 0.19-20.29 mg/g, respectively. Among the nucleosides studied, cordycepin was characteristically included in Cordyceps militaris (L.) Link. (CM), which is one of key Cordyceps allies, and might be a good marker for authenticating CM. The ratio of nucleosides to adenosine contents in Cordyceps seemed to be a useful marker for authentication and quality control of Cordyceps. Copyright (C) 2008 John Wiley & Sons, Ltd. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINE ; CULTURED CORDYCEPS ; FRUITING BODY ; SINENSIS ; MILITARIS ; CELLS ; BASES ; EXTRACTION ; MYCELIUM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000256875200011 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272902]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Nakashima, Kenichiro |
| 作者单位 | 1.Nagasaki Univ, Grad Sch Biomed Sci, Div Analyt Res Pharmacoinformat, Dept Clin Pharm,Course Pharmaceut Sci, Nagasaki 8528521, Japan; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Metab & Pharmacokinet Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ikeda, Rie,Nishimura, Miho,Sun, Yen,et al. Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(6):630-636. |
| APA | Ikeda, Rie,Nishimura, Miho,Sun, Yen,Wada, Mitsuhiro,&Nakashima, Kenichiro.(2008).Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies.BIOMEDICAL CHROMATOGRAPHY,22(6),630-636. |
| MLA | Ikeda, Rie,et al."Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies".BIOMEDICAL CHROMATOGRAPHY 22.6(2008):630-636. |
入库方式： OAI收割
来源：上海药物研究所
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