Arraying proteins by cell-free synthesis
文献类型：期刊论文
| 作者 | He, Mingyue; Wang, Ming-Wei
|
| 刊名 | BIOMOLECULAR ENGINEERING
 |
| 出版日期 | 2007-10 |
| 卷号 | 24期号:4页码:375-380 |
| 关键词 | protein microarray cell-free technologies transcription expression on chip synthesis |
| ISSN号 | 1389-0344 |
| DOI | 10.1016/j.bioeng.2007.05.002 |
| 文献子类 | Review |
| 英文摘要 | Recent advances in life science have led to great motivation for the development of protein arrays to study functions of genome-encoded proteins. While traditional cell-based methods have been commonly used for generating protein arrays, they are usually a time-consuming process with a number of technical challenges. Cell-free protein synthesis offers an attractive system for making protein arrays, not only does it rapidly converts the genetic information into functional proteins without the need for DNA cloning, but also presents a flexible environment amenable to production of folded proteins or proteins with defined modifications. Recent advancements have made it possible to rapidly generate protein arrays from PCR DNA templates through parallel on-chip protein synthesis. This article reviews current cell-free protein array technologies and their proteomic applications. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | IN-VITRO TRANSLATION ; FREE EXPRESSION ; SYNTHESIS SYSTEM ; MESSENGER-RNA ; MICROARRAYS ; TECHNOLOGY ; GENERATION ; FUSION ; DNA ; IMMOBILIZATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000250243900002 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273136]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | He, Mingyue |
| 作者单位 | 1.Babraham Inst, Technol Res Grp, Cambridge CB22 3AT, England 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Mingyue,Wang, Ming-Wei. Arraying proteins by cell-free synthesis[J]. BIOMOLECULAR ENGINEERING,2007,24(4):375-380. |
| APA | He, Mingyue,&Wang, Ming-Wei.(2007).Arraying proteins by cell-free synthesis.BIOMOLECULAR ENGINEERING,24(4),375-380. |
| MLA | He, Mingyue,et al."Arraying proteins by cell-free synthesis".BIOMOLECULAR ENGINEERING 24.4(2007):375-380. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。