Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate
文献类型：期刊论文
| 作者 | Li, Yiming; Yin, Guowei; Wei, Wei; Wang, Hengbin; Jiang, Shanhao; Zhu, Dayuan; Du, Weihong |
| 刊名 | BIOPHYSICAL CHEMISTRY
 |
| 出版日期 | 2007-09 |
| 卷号 | 129期号:2-3页码:212-217 |
| 关键词 | acetyleholinesterase Lycopodium alkaloids H-1 spin-lattice relaxation rate ligand-macromolecules interaction |
| ISSN号 | 0301-4622 |
| DOI | 10.1016/j.bpc.2007.05.020 |
| 文献子类 | Article |
| 英文摘要 | In order to further understand the interaction processes between the Lycopodium alkaloids and acetylcholinesterase, the binding properties of N-acetyl Imperzine A (1), Imperzine B (2) and Imperzine F (3) with Torpediniforms Nacline acetylcholinesterase (TnAchE) were investigated by H-1 NMR methods. The nonselective, selective and double-selective spin-lattice relaxation rates were acquired in the absence and presence of TnAchE at a ratio of [ligand]/[protein] = 1:0.005. The selective relaxation rates show protons of 1-3 have dipole-dipole interaction with protons of TnAchE at the binding interface. The molecular rotational correlation time of bound ligands was calculated by double-selective relaxation rate at 298 K, which showed that 1-3 had high affinity with the protein. The results indicate that investigation of H-1 NMR relaxation data is a useful method to locate the new Lycopodium alkaloids as AchE inhibitors. C 2007 Elsevier B.V All rights reserved. |
| WOS关键词 | TORPEDO-CALIFORNICA ACETYLCHOLINESTERASE ; SELECTIVE EXCITATION ; CROSS-RELAXATION ; HUPERZINE-A ; NMR ; INHIBITORS ; BINDING ; LIGANDS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000249151100012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273156]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhu, Dayuan |
| 作者单位 | 1.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yiming,Yin, Guowei,Wei, Wei,et al. Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate[J]. BIOPHYSICAL CHEMISTRY,2007,129(2-3):212-217. |
| APA | Li, Yiming.,Yin, Guowei.,Wei, Wei.,Wang, Hengbin.,Jiang, Shanhao.,...&Du, Weihong.(2007).Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate.BIOPHYSICAL CHEMISTRY,129(2-3),212-217. |
| MLA | Li, Yiming,et al."Interactions of Lycopodium alkaloids with acetylcholine sterase investigated by H-1 NMR relaxation rate".BIOPHYSICAL CHEMISTRY 129.2-3(2007):212-217. |
入库方式： OAI收割
来源：上海药物研究所
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