A chemiluminescence-based assessment of androgen-binding activity in a large pedigree affected with androgen insensitivity syndrome
文献类型：期刊论文
| 作者 | Wang, Jiu-Cun; Wang, Minghua; Zhang, Rongmei; Zhao, Zhimin; Chen, Jie; Luo, Zewei |
| 刊名 | LUMINESCENCE
 |
| 出版日期 | 2007-07 |
| 卷号 | 22期号:4页码:370-378 |
| 关键词 | chemiluminescence ligand-binding assay androgen receptor genital skin fibroblast androgen insensitivity syndrome |
| ISSN号 | 1522-7235 |
| DOI | 10.1002/bio.973 |
| 文献子类 | Article |
| 英文摘要 | We report a novel chemiluminescence (CL)-based method for assaying the ligand-binding activity of tile androgen receptor. The central parts of this method are the utilization of the steroid CL marker as the replacement of the radioactive label in the conventional ligand-binding assay and the determination of the binding activity by the light measurement of tile bound CL-label under an H2O7-microperoxidase system. The properties and reliability of this assay were investigated and verified using genital skin fibroblasts (GSF) from seven normal males. The method is precise (CV < 7% for both B-max and K-d) with high correlation coefficients (r > 0.93) in each Scatchard linear regression analysis. This assay can determine tile androgen binding properties using only a quarter of the cells (approximately 40 000 cells/data point) of that required by the radiolabelling approach. The utility of the method was illustrated by binding experiment on the GSFs of several patients from a large Chinese family affected with androgen insensitivity syndrome. The familial distinct feature is that all patients shared an identical Arg840Cys substitution in the androgen receptor but displayed high phenotypic variation in disorders of male sexual development. The patients selected for the present study represent a wide spectrum of this phenotypic variation. This study thus provides insights on the pleiotropic effects of the mutation. In conclusion, the CL-based method can serve as an effective, precise and reliable replacement for the racholabelling approach and has the advantages of simplicity, cost-effectiveness and health and environmental safety over tile counterpart. Copyright (C) 2007 John Wiley & Sons, Ltd. |
| WOS关键词 | RECEPTOR DEFECTS ; RESISTANCE ; DOMAIN ; STABILIZATION ; IMMUNOASSAYS ; FIBROBLASTS ; MUTATIONS ; STABILITY |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000249139200014 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273217]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Luo, Zewei |
| 作者单位 | 1.Fudan Univ, Inst Genet, Sch Life Sci, Chinas State Key Lab Genet Engn, Shanghai 200433, Peoples R China 2.Fudan Univ, Morgan Tan Int Ctr Life Sci MTIC, Shanghai 200433, Peoples R China 3.Guiyang Tradit Chinese Med Coll, Affiliated Hosp 1, Dept Surg, Guiyang, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China 5.Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England |
| 推荐引用方式 GB/T 7714 | Wang, Jiu-Cun,Wang, Minghua,Zhang, Rongmei,et al. A chemiluminescence-based assessment of androgen-binding activity in a large pedigree affected with androgen insensitivity syndrome[J]. LUMINESCENCE,2007,22(4):370-378. |
| APA | Wang, Jiu-Cun,Wang, Minghua,Zhang, Rongmei,Zhao, Zhimin,Chen, Jie,&Luo, Zewei.(2007).A chemiluminescence-based assessment of androgen-binding activity in a large pedigree affected with androgen insensitivity syndrome.LUMINESCENCE,22(4),370-378. |
| MLA | Wang, Jiu-Cun,et al."A chemiluminescence-based assessment of androgen-binding activity in a large pedigree affected with androgen insensitivity syndrome".LUMINESCENCE 22.4(2007):370-378. |
入库方式： OAI收割
来源：上海药物研究所
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