中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Intein-mediated expression is an effective approach in the study of beta-defensins

文献类型:期刊论文

作者Diao, Hua; Guo, Chenyun; Lin, Donghai; Zhang, Yonglian
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2007-06-15
卷号357期号:4页码:840-846
关键词intein beta-defensin defensin fusion-expression host defense innate immunity antimicrobial activity
ISSN号0006-291X
DOI10.1016/j.bbrc.2007.03.149
文献子类Article
英文摘要Mammalian beta-defensins are an important family of host defense peptides with diverse functions. Surprisingly most of the mammalian beta-defensin genes are revealed preferentially expressed in the male organs. There is a pressing need to understand how the ample defensin repertoires work in both host defense and fertility with an aim to overcome antibiotic resistance of pathogens and reproductive problems. The biggest obstacle is the production of beta-defensin peptides as P-defensins are small, antimicrobial and multi-disultide molecules. In this study, the well documented HBD2, function-unknown RBD1 and function-partly-known rBin1b are successfully expressed and assayed. This approach overcomes the difficulties in beta-defensin production and provides a convenient and economical peptide-production platform to elucidate the antimicrobial activities and clinical prospects of P-defensins. In the strategy of recombinant expression, this approach may be the best to develop the "natural" peptide pools for both host defense and fertility in a cost-effective manner. (c) 2007 Elsevier Inc. All rights reserved.
WOS关键词ESCHERICHIA-COLI ; HUMAN BETA-DEFENSIN-2 ; CANDIDA-ALBICANS ; ANTIMICROBIAL PEPTIDES ; PROTEIN-PURIFICATION ; RECOMBINANT PROTEINS ; INNATE ; GENE ; ANTIBIOTICS ; CLEAVAGE
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000246648400004
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/273223]  
专题中国科学院上海药物研究所
通讯作者Zhang, Yonglian
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Key Lab Mol Androl, State Key Lab Mol Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
2.Shanghai Inst Planned Parenthood Res, Shanghai 200032, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Diao, Hua,Guo, Chenyun,Lin, Donghai,et al. Intein-mediated expression is an effective approach in the study of beta-defensins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,357(4):840-846.
APA Diao, Hua,Guo, Chenyun,Lin, Donghai,&Zhang, Yonglian.(2007).Intein-mediated expression is an effective approach in the study of beta-defensins.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,357(4),840-846.
MLA Diao, Hua,et al."Intein-mediated expression is an effective approach in the study of beta-defensins".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 357.4(2007):840-846.

入库方式: OAI收割

来源:上海药物研究所

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