Silencing of heparanase by siRNA inhibits tumor metastasis and angiogenesis of human breast cancer in vitro and in vivo
文献类型：期刊论文
| 作者 | Zhang, Zhong-Hua; Chen, Yi ; Zhao, Hua-Jun; Xie, Cheng-Ying; Ding, Jian; Hou, Yong-Tai
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| 刊名 | CANCER BIOLOGY & THERAPY
 |
| 出版日期 | 2007-04 |
| 卷号 | 6期号:4页码:587-595 |
| 关键词 | RNA interference siRNA human heparanase tumor metastasis angiogenesis human breast cancer gene silence |
| ISSN号 | 1538-4047 |
| DOI | 10.4161/cbt.6.4.3888 |
| 文献子类 | Article |
| 英文摘要 | Expression of the heparanase gene is associated with invasive, angiogenic and metastatic potential of diverse malignant tumors and cell lines. Here we used RNA interference strategies to evaluate the role of human heparanase in breast malignancy and to explore the therapeutic potential of its specific targeting. The siRNA targeting human heparanase almost completely inhibited the expression of heparanase in human breast carcinoma MDA-MB-435 cells, whereas the mismatched siRNA showed no effect. Cells transfected with heparanase siRNA expressed significantly less heparanase and profoundly reduced invasion and adhesion in vitro. In MDA-MB-435 cell xenograft model, tumors treated with siRNA were less vascularized and less metastatic than those treated with saline and the mismatched controls. The association of reduced levels of heparanase and altered tumorigenic properties in cells with anti-heparanase siRNA indicates that heparanase is important in cancer progress and has potential use as a target for anticancer drug development. |
| WOS关键词 | ENDOTHELIAL GROWTH-FACTOR ; GENE-THERAPY ; NUDE-MICE ; E1A GENE ; EXPRESSION ; SULFATE ; CLONING ; PURIFICATION ; LYSOSOMES ; SECRETION |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000248102700022 |
| 出版者 | LANDES BIOSCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273291]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Hou, Yong-Tai |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Materia Med, Div Mol Pharmacol, Shanghai 210203, Peoples R China 2.Chinese Acad Sci, Div Anti Tumor Pharmacol, Shanghai 210203, Peoples R China 3.Chinese Acad Sci, Grad Sch, Shanghai 210203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhong-Hua,Chen, Yi,Zhao, Hua-Jun,et al. Silencing of heparanase by siRNA inhibits tumor metastasis and angiogenesis of human breast cancer in vitro and in vivo[J]. CANCER BIOLOGY & THERAPY,2007,6(4):587-595. |
| APA | Zhang, Zhong-Hua,Chen, Yi,Zhao, Hua-Jun,Xie, Cheng-Ying,Ding, Jian,&Hou, Yong-Tai.(2007).Silencing of heparanase by siRNA inhibits tumor metastasis and angiogenesis of human breast cancer in vitro and in vivo.CANCER BIOLOGY & THERAPY,6(4),587-595. |
| MLA | Zhang, Zhong-Hua,et al."Silencing of heparanase by siRNA inhibits tumor metastasis and angiogenesis of human breast cancer in vitro and in vivo".CANCER BIOLOGY & THERAPY 6.4(2007):587-595. |
入库方式： OAI收割
来源：上海药物研究所
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