Microbial transformation of dextromethorphan by Cunninghamella blakesleeana AS 3.153
文献类型：期刊论文
| 作者 | Lin, Lihong; Huang, Haihua; Zhang, Peng; Qi, Xiulan; Zhong, Dafang
|
| 刊名 | CHEMICAL & PHARMACEUTICAL BULLETIN
 |
| 出版日期 | 2007-04 |
| 卷号 | 55期号:4页码:658-661 |
| 关键词 | Cunninghamella blakesleeana microbial transformation dextromethorphan cytochrome P450 2D6 liquid chromatography-tandem mass spectrometry |
| ISSN号 | 0009-2363 |
| DOI | 10.1248/cpb.55.658 |
| 文献子类 | Article |
| 英文摘要 | The capability of Cunninghamella blakesleeana AS 3.153 to transform CYP2D6 probe drug dextromethorphan was investigated. Metabolites produced by strain AS 3.153 were detected by liquid chromatography-tandem mass spectrometry (LC-MSn) and the metabolite dextrorphan was identified by reference to confirm its structure. The yield of dextrorphan produced by C. blakesleeana AS 3.153 was over 90%. Quinidine, a CYP2D6 selective inhibitor, was applied to investigate its effect on biotransformation. The concentration of quinidine was 4-folds higher than that of dextromethorphan and the yield of dextrorphan was reduced by 84%, which proved there was drug metabolism enzyme similar to CYP2D6 in C. blakesleeana AS 3.153. It is concluded that C. blakesleeana AS 3.153 can be used as the suitable model strain in vitro to mimic human CYP2D6 metabolism. |
| WOS关键词 | METABOLISM ; BIOTRANSFORMATION |
| WOS研究方向 | Pharmacology & Pharmacy ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000246377800031 |
| 出版者 | PHARMACEUTICAL SOC JAPAN |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273292]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Huang, Haihua |
| 作者单位 | 1.Shanghai Drug Metab Biotech Ltd, Shanghai 201204, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Lihong,Huang, Haihua,Zhang, Peng,et al. Microbial transformation of dextromethorphan by Cunninghamella blakesleeana AS 3.153[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,2007,55(4):658-661. |
| APA | Lin, Lihong,Huang, Haihua,Zhang, Peng,Qi, Xiulan,&Zhong, Dafang.(2007).Microbial transformation of dextromethorphan by Cunninghamella blakesleeana AS 3.153.CHEMICAL & PHARMACEUTICAL BULLETIN,55(4),658-661. |
| MLA | Lin, Lihong,et al."Microbial transformation of dextromethorphan by Cunninghamella blakesleeana AS 3.153".CHEMICAL & PHARMACEUTICAL BULLETIN 55.4(2007):658-661. |
入库方式： OAI收割
来源：上海药物研究所
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