H-1 NMR relaxation investigation of huperzine E binding to acetylcholineste
文献类型：期刊论文
| 作者 | Du Wei-Hong; Li Yi-Ming; Gan Qiu-Ling; Jiang Shan-Hao; Tan Chang-Heng; Zhu Da-Yuan |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2007-03-14 |
| 卷号 | 65期号:5页码:415-420 |
| 关键词 | huperzine E acetylcholinesterase proton spin-lattice relaxation rate molecular motional correlation time NMR |
| ISSN号 | 0567-7351 |
| 其他题名 | 应用~1H NMR弛豫观察石杉碱戊与乙酰胆碱酯酶的结合 |
| 文献子类 | Article |
| 英文摘要 | In order to search for better acetylcholinesterase (AchE) inhibitors, the binding properties of AchE with huperizine E, which is a derivative of huperzine A, were investigated by H-1 NMR methods. The nonselective, selective and double-selective spin-lattice relaxation rates of some protons in huperzine E were acquired in the absence and presence of AchE at a concentration ratio of [ligand] : [protein] = 1 : 0.005. The enhancements of selective relaxation rates of these protons were obvious after adding AchE. The molecular motional correlation times of two pairs of protons, H-1a/H-1b and H-2/H-3, in the bound state at T=298 K were 11.7 and 9.46 ns respectively, while they were 27.7 and 35.2 ps in the free state. All of these show that Huperzine E has high binding affinity with AchE. |
| WOS关键词 | DERIVATIVES |
| 资助项目 | National Natural Science Foundation of China[90409015] ; National Natural Science Foundation of China[20473013] |
| WOS研究方向 | Chemistry |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2757226 |
| WOS记录号 | WOS:000245142900008 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273305]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Du Wei-Hong |
| 作者单位 | 1.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du Wei-Hong,Li Yi-Ming,Gan Qiu-Ling,et al. H-1 NMR relaxation investigation of huperzine E binding to acetylcholineste[J]. ACTA CHIMICA SINICA,2007,65(5):415-420. |
| APA | Du Wei-Hong,Li Yi-Ming,Gan Qiu-Ling,Jiang Shan-Hao,Tan Chang-Heng,&Zhu Da-Yuan.(2007).H-1 NMR relaxation investigation of huperzine E binding to acetylcholineste.ACTA CHIMICA SINICA,65(5),415-420. |
| MLA | Du Wei-Hong,et al."H-1 NMR relaxation investigation of huperzine E binding to acetylcholineste".ACTA CHIMICA SINICA 65.5(2007):415-420. |
入库方式： OAI收割
来源：上海药物研究所
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