Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats
文献类型：期刊论文
| 作者 | Zhang, YY; Zhu, HY; Ye, G; Huang, CG ; Yang, YZ; Chen, RZ; Yu, Y; Cui, XL
|
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2006-03-20 |
| 卷号 | 78期号:17页码:1998-2001 |
| 关键词 | sophoridine coxsackievirus B3 viral myocarditis pharmacokinetics cytokines |
| ISSN号 | 0024-3205 |
| DOI | 10.1016/j.lfs.2005.09.034 |
| 文献子类 | Article |
| 英文摘要 | Coxsackievirus 133 (CVB3) is a major pathogen for acute and chronic viral myocarditis. The aim of this study was to investigate the antiviral effects of sophoridine, an alkaloid extracted from Chinese medicinal herb, Sophora flavescens, against CVB3, and the underlying pharmacokinetics. First, we determined the antiviral effects of sophoridine against CVB3 in in vitro (primarily cultured myocardial cells), in vivo (BALB/c mice) and serum pharmacological experiments. Then, we determined the pharmacokinetic behavior in serum samples of SD rats after oral administration by HPLC. Finally, we determined the effects of sophoridine on the production of cytokines in a murine viral myocarditis model by measuring mRNA expression of some important cytokines in hearts of infected BALB/c mice by RT-PCR. We found that sophoridine exhibited obvious antiviral effects both in vitro and in vivo, and serum samples obtained from rats with oral administration of sophoridine reduced the virus titers in infected myocardial cells. The serum concentration profile correlated closely with antiviral activity profile. Moreover, sophoridine significantly enhanced mRNA expression of IL-10 and IFN-gamma, but decreased TNF-alpha mRNA expression. In conclusion, sophoridine possesses antiviral activities against CVB3, by regulating cytokine expression, and it is likely that sophoridine itself, not its metabolites, is mainly responsible for the antiviral activities. Therefore, sophoridine may represent a potential therapeutic agent for viral myocarditis. (c) 2005 Elsevier Inc. All rights reserved. |
| WOS关键词 | VIRAL MYOCARDITIS ; DNA VACCINE ; CARDIOMYOPATHY ; PROTECTION ; CHALLENGE ; CELLS ; MICE |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000236307200014 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273641]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Huang, CG |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai, Peoples R China 2.Fudan Univ, Zhongshan Hosp, Inst Cardiovasc Dis, Shanghai 200433, Peoples R China 3.Chinese Acad Tradit Chinese Med, Inst Trad Chinese Med, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YY,Zhu, HY,Ye, G,et al. Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats[J]. LIFE SCIENCES,2006,78(17):1998-2001. |
| APA | Zhang, YY.,Zhu, HY.,Ye, G.,Huang, CG.,Yang, YZ.,...&Cui, XL.(2006).Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats.LIFE SCIENCES,78(17),1998-2001. |
| MLA | Zhang, YY,et al."Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats".LIFE SCIENCES 78.17(2006):1998-2001. |
入库方式： OAI收割
来源：上海药物研究所
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