Liquid chromatography-negative ion electrospray tandem mass spectrometry method for the quantification of ezetimibe in human plasma
文献类型：期刊论文
| 作者 | Li, SJ; Liu, GY; Jia, JY; Li, XC; Yu, C |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2006-03-03 |
| 卷号 | 40期号:4页码:987-992 |
| 关键词 | ezetimibe LC-MS/MS negative electrospray ionization |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2005.07.053 |
| 文献子类 | Article |
| 英文摘要 | A simple, reliable and sensitive liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed and validated for quantification of free and total ezetimibe in human plasma. The analyte and internal standard ((13)C6-ezetimibe) were extracted by liquid-liquid extraction with methyl tert-butyl ether. The reversed-phase chromatographic separation was performed on a Capcell C18 column, and the plasma extract was eluted with a gradient consisting of acetonitrile and 5 mM ammonium acetate. The analyte was detected using negative ionization by multiple reaction monitoring mode. The mass transition pairs of m/z 408.5 -> 270.8 and m/z 414.5 -> 276.8 were used to detect ezetimibe and internal standard, respectively. The assay exhibited linear ranges from 0.02 to 20 ng/ml for free ezetimibe and 0.25 to 250 ng/ml for total czetimibe in human plasma. Acceptable precision and accuracy were obtained for concentrations of the calibration standard and quality control. The validated method was successfully used to analyze human plasma samples for application in a pharmacokinetic study. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS关键词 | CHOLESTEROL ABSORPTION INHIBITOR ; PRIMARY HYPERCHOLESTEROLEMIA ; POOLED ANALYSIS ; DOUBLE-BLIND ; EFFICACY ; SAFETY ; TOLERABILITY ; MULTICENTER ; POTENT |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000236344100024 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273654]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yu, C |
| 作者单位 | 1.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Dept Pharmacol, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, SJ,Liu, GY,Jia, JY,et al. Liquid chromatography-negative ion electrospray tandem mass spectrometry method for the quantification of ezetimibe in human plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2006,40(4):987-992. |
| APA | Li, SJ,Liu, GY,Jia, JY,Li, XC,&Yu, C.(2006).Liquid chromatography-negative ion electrospray tandem mass spectrometry method for the quantification of ezetimibe in human plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,40(4),987-992. |
| MLA | Li, SJ,et al."Liquid chromatography-negative ion electrospray tandem mass spectrometry method for the quantification of ezetimibe in human plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 40.4(2006):987-992. |
入库方式： OAI收割
来源：上海药物研究所
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