Activation of insulin-like growth factor I receptor-mediated pathway by ginsenoside Rg1
文献类型：期刊论文
| 作者 | Chen, WF; Lau, WS; Cheung, PY; Guo, DA ; Wong, MS
|
| 刊名 | BRITISH JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2006-03 |
| 卷号 | 147期号:5页码:542-551 |
| 关键词 | ginsenoside Rg1 estrogen insulin-like growth factor I receptor human breast cancer phytoestrogen |
| ISSN号 | 0007-1188 |
| DOI | 10.1038/sj.bjp.0706640 |
| 文献子类 | Article |
| 英文摘要 | 1 Ginsenoside Rg1, an active ingredient in ginseng, was previously shown to be a novel class of potent phytoestrogen. The present study aims at investigating the molecular mechanisms involved in mediating its actions in human breast cancer (MCF-7) cells. 2 Rg1 ( 1 pM) stimulates cell proliferation (P<0.01) and estrogen-responsive pS2 mRNA expression (P<0.05) without alteration of estrogen receptor alpha (ER alpha) protein or mRNA expression in MCF-7 cells. In addition, 10(-1)4 - 10(-4) M of Rg1 does not demonstrate specific binding to ER alpha. 3 We hypothesize that Rg1 may exert its actions in MCF-7 cell via the activation of crosstalk between ER- and insulin growth factor I receptor (IGF-IR)- dependent pathways. 4 The results indicate that Rg1 significantly increases IGF-IR expression and IGF-IR promoter activity in MCF-7 cells (P<0.05). Cotreatment of MCF-7 cells with 1 mu M of estrogen antagonist ICI 182,780 completely abolishes the effects of Rg1 on IGF-IR expression. 5 Furthermore, Rg1 enhances tyrosine phosphorylation of IRS-1 in MCF-7 cells upon IGF-I stimulation and the activation of IRS-1 phosphorylation is also ER- dependent. 6 Taken together, our results suggest that Rg1 not only increases IGF-IR expression but also enhances IGF-IR- mediated signaling pathways in MCF-7 cells. The stimulation of IGF-IR expression by Rg1 in MCF-7 cells appears to require ER, and its actions might involve ligand-independent activation of ER. |
| WOS关键词 | BREAST-CANCER CELLS ; ESTROGEN-RECEPTOR ; PROTEIN-KINASE ; MENOPAUSAL SYMPTOMS ; SIGNALING PATHWAYS ; ENDOTHELIAL-CELLS ; PANAX-GINSENG ; MCF-7 CELLS ; ALPHA ; MECHANISM |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000236170200010 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273656]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wong, MS |
| 作者单位 | 1.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Inst Mol Technol Drug Discovery & Synth, Cent Lab, Kowloon, Hong Kong, Peoples R China 2.Qingdao Univ, Coll Med, Dept Physiol, Qingdao 266021, Peoples R China 3.State Key Lab Chinese Med & Mol Pharmacol, Shenzhen 518057, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, WF,Lau, WS,Cheung, PY,et al. Activation of insulin-like growth factor I receptor-mediated pathway by ginsenoside Rg1[J]. BRITISH JOURNAL OF PHARMACOLOGY,2006,147(5):542-551. |
| APA | Chen, WF,Lau, WS,Cheung, PY,Guo, DA,&Wong, MS.(2006).Activation of insulin-like growth factor I receptor-mediated pathway by ginsenoside Rg1.BRITISH JOURNAL OF PHARMACOLOGY,147(5),542-551. |
| MLA | Chen, WF,et al."Activation of insulin-like growth factor I receptor-mediated pathway by ginsenoside Rg1".BRITISH JOURNAL OF PHARMACOLOGY 147.5(2006):542-551. |
入库方式： OAI收割
来源：上海药物研究所
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