Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling
文献类型：期刊论文
| 作者 | Yao, G; Chen, WY; Luo, HB; Jiang, QF; Xia, ZX; Zang, L; Zuo, JP ; Wei, X; Chen, ZJ; Shen, X
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| 刊名 | INTERNATIONAL IMMUNOLOGY
 |
| 出版日期 | 2006-01 |
| 卷号 | 18期号:1页码:19-29 |
| 关键词 | 11B.11 core functional region of IL-4 IL-4 molecular modeling peptide phage display |
| ISSN号 | 0953-8178 |
| DOI | 10.1093/intimm/dxh338 |
| 文献子类 | Article |
| 英文摘要 | Murine IL-4 is a pleiotropic cytokine with undefined core functional region for eliciting downstream signaling. We used molecular modeling to predict the binding sites recognized by an anti-IL-4-neutralizing mAb (11B.11) and peptide phage display to delineate their makeup. The results of these approaches were confirmed by site-directed mutagenesis analysis. The results suggest that the amino acid residues spanning from 79 to 86 (QRLFRAFR) on IL-4 are of the major binding site for 11B.11. Furthermore, the functional experiments demonstrate that the residues R80, R83 and R86, which are located in the helix C of murine IL-4, play a crucial role in binding to the IL-4R alpha-chain. Taken together, a new core functional region of murine IL-4 is identified, which provides new insight into the interaction between IL-4 and IL-4R alpha. In addition, the results demonstrate that 11B.11 binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor. |
| WOS关键词 | AMINO-ACID-RESIDUES ; MONOCLONAL-ANTIBODY ; HUMAN INTERLEUKIN-4 ; SIGNAL-TRANSDUCTION ; IN-VIVO ; MOUSE INTERLEUKIN-4 ; CRYSTAL-STRUCTURE ; RECEPTOR-BINDING ; SWISS-MODEL ; GAMMA-CHAIN |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000234436500003 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273734]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Sun, B |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 201203, Peoples R China 4.GL Biochem Shanghai Ltd, Shanghai 201203, Peoples R China 5.Univ Texas, MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA 6.Shanghai Univ E Inst, Div Immunol, Shanghai 200041, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, G,Chen, WY,Luo, HB,et al. Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling[J]. INTERNATIONAL IMMUNOLOGY,2006,18(1):19-29. |
| APA | Yao, G.,Chen, WY.,Luo, HB.,Jiang, QF.,Xia, ZX.,...&Sun, B.(2006).Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling.INTERNATIONAL IMMUNOLOGY,18(1),19-29. |
| MLA | Yao, G,et al."Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling".INTERNATIONAL IMMUNOLOGY 18.1(2006):19-29. |
入库方式： OAI收割
来源：上海药物研究所
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