Changes in exercise-induced gene expression in 5 '-AMP-activated protein kinase gamma 3-null and gamma 3 R225Q transgenic mice
文献类型：期刊论文
| 作者 | Barnes, BR; Long, YC; Steiler, TL; Leng, Y ; Galuska, D; Wojtaszewski, JFP; Andersson, L; Zierath, JR
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| 刊名 | DIABETES
 |
| 出版日期 | 2005-12 |
| 卷号 | 54期号:12页码:3484-3489 |
| ISSN号 | 0012-1797 |
| DOI | 10.2337/diabetes.54.12.3484 |
| 文献子类 | Article |
| 英文摘要 | 5'-AMP-activated protein kinase (AMPK) is important for metabolic sensing. We used AMPK gamma 3 mutant-overexpressing Tg-Prkag3(225Q) and AMPK gamma 3-knockout Prkag3(-/-) mice to determine the role of the AMPK gamma 3 isoform in exercise-induced metabolic and gene regulatory responses in skeletal muscle. Mice were studied after 2 h swimming or 2.5 h recovery. Exercise increased basal and insulin-stimulated glucose transport, with similar responses among genotypes. In Tg-Prkag3(225Q) mice, acetyl-CoA carboxylase (ACC) phosphorylation was increased and triglyceride content was reduced after exercise, suggesting that this mutation promotes greater reliance on lipid oxidation. In contrast, ACC phosphorylation and triglyceride content was similar between wild-type and Prkag3(-/-) mice. Expression of genes involved in lipid and glucose metabolism was altered by genetic modification of AMPK gamma 3. Expression of lipoprotein lipase 1, carnitine palmitoyl transferase 1b, and 3-hydroxyacyl-CoA dehydrogenase was increased in Tg-Prkag3(225Q) mice, with opposing effects in Prkag3(-/-) mice after exercise. GLUT4, hexokinase 11 (HKII), and glycogen synthase mRNA expression was increased in Tg-Prkag3(225Q) mice after exercise. GLUT4 and HKII mRNA expression was increased in wild-type mice and blunted in Prkag3(-/-) mice after recovery. In conclusion, the Prkag3(225Q) mutation, rather than presence of a functional AMPK gamma 3 isoform, directly promotes metabolic and gene regulatory responses along lipid oxidative pathways in skeletal muscle after endurance exercise. |
| WOS关键词 | GLUCOSE-TRANSPORT CAPACITY ; HUMAN SKELETAL-MUSCLE ; GLYCOGEN-SYNTHASE ; ACTIVATION ; INCREASES ; INSULIN ; AMPK ; STIMULATION ; METABOLISM ; STORAGE |
| WOS研究方向 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000233582500018 |
| 出版者 | AMER DIABETES ASSOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273761]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zierath, JR |
| 作者单位 | 1.Swedish Univ Agr Sci, Uppsala Biomed Ctr, Dept Anim Breeding & Genet, Uppsala, Sweden 2.Karolinska Inst, Dept Surg Sci, Sect Integrat Physiol, S-17177 Stockholm, Sweden 3.Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 5.Univ Copenhagen, Inst Exercise & Sport Sci, Copenhagen Muscle Res Ctr, Dept Human Physiol, Copenhagen, Denmark 6.Uppsala Univ, Uppsala Biomed Ctr, Dept Med Biochem & Microbiol, Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Barnes, BR,Long, YC,Steiler, TL,et al. Changes in exercise-induced gene expression in 5 '-AMP-activated protein kinase gamma 3-null and gamma 3 R225Q transgenic mice[J]. DIABETES,2005,54(12):3484-3489. |
| APA | Barnes, BR.,Long, YC.,Steiler, TL.,Leng, Y.,Galuska, D.,...&Zierath, JR.(2005).Changes in exercise-induced gene expression in 5 '-AMP-activated protein kinase gamma 3-null and gamma 3 R225Q transgenic mice.DIABETES,54(12),3484-3489. |
| MLA | Barnes, BR,et al."Changes in exercise-induced gene expression in 5 '-AMP-activated protein kinase gamma 3-null and gamma 3 R225Q transgenic mice".DIABETES 54.12(2005):3484-3489. |
入库方式： OAI收割
来源：上海药物研究所
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