Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle
文献类型：期刊论文
| 作者 | Long, YC; Barnes, BR; Mahlapuu, M; Steiler, TL; Martinsson, S; Leng, Y ; Wallberg-Henriksson, H; Andersson, L; Zierath, JR
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| 刊名 | DIABETOLOGIA
 |
| 出版日期 | 2005-11 |
| 卷号 | 48期号:11页码:2354-2364 |
| 关键词 | AMP-activated protein kinase fasting metabolism skeletal muscle transcription |
| ISSN号 | 0012-186X |
| DOI | 10.1007/s00125-005-1962-5 |
| 文献子类 | Article |
| 英文摘要 | Aims/hypothesis: AMP-activated protein kinase (AMPK) regulates metabolic adaptations in skeletal muscle. The aim of this study was to investigate whether AMPK modulates the expression of skeletal muscle genes that have been implicated in lipid and glucose metabolism under fed or fasting conditions. Methods: Two genetically modified animal models were used: AMPK gamma 3 subunit knockout mice (Prkag3(-/-)) and skeletal muscle-specific transgenic mice (Tg-Prkag3225Q) that express a mutant (R225Q) gamma 3 subunit. Levels of mRNA transcripts of genes involved in lipid and glucose metabolism in white gastrocnemius muscles of these mice (under fed or 16-h fasting conditions) were assessed by quantitative real-time PCR. Results: Wild-type mice displayed a coordinated increase in the transcription of skeletal muscle genes encoding proteins involved in lipid/oxidative metabolism (lipoprotein lipase, fatty acid transporter, carnitine palmitoyl transferase-1 and citrate synthase) and glucose metabolism (glycogen synthase and lactate dehydrogenase) in response to fasting. In contrast, these fasting-induced responses were impaired in Prkag3(-/-) mice. The transcription of genes involved in lipid and oxidative metabolism was increased in the skeletal muscle of Tg-Prkag3225Q mice compared with that in wild-type mice. Moreover, the expression of the genes encoding hexokinase II and 6-phosphofrucktokinase was decreased in Tg-Prkag3225Qsupercript stop mice after fasting. Conclusions/interpretation: AMPK is involved in the coordinated transcription of genes critical for lipid and glucose metabolism in white glycolytic skeletal muscle. |
| WOS关键词 | PYRUVATE-DEHYDROGENASE COMPLEX ; UNCOUPLING PROTEIN-3 ; ENZYME-ACTIVITIES ; GLYCOGEN-CONTENT ; CARRIER PIGS ; FATTY-ACIDS ; INCREASES ; OXIDATION ; INSULIN ; MICE |
| WOS研究方向 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000233248200024 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273782]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zierath, JR |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Karolinska Inst, Dept Surg Sci, Sect Integrat Physiol, S-17177 Stockholm, Sweden 3.Arexis, Gothenburg, Sweden 4.Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Uppsala, Sweden 5.Uppsala Univ, Uppsala Biomed Ctr, Dept Med Biochem & Microbiol, Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Long, YC,Barnes, BR,Mahlapuu, M,et al. Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle[J]. DIABETOLOGIA,2005,48(11):2354-2364. |
| APA | Long, YC.,Barnes, BR.,Mahlapuu, M.,Steiler, TL.,Martinsson, S.,...&Zierath, JR.(2005).Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle.DIABETOLOGIA,48(11),2354-2364. |
| MLA | Long, YC,et al."Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle".DIABETOLOGIA 48.11(2005):2354-2364. |
入库方式： OAI收割
来源：上海药物研究所
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