Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli
文献类型：期刊论文
| 作者 | Ge, BS; Tang, ZH; Lin, LP; Ren, YH; Yang, Y ; Qin, S
|
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2005-06 |
| 卷号 | 27期号:11页码:783-787 |
| 关键词 | allophycocyanin Escherichia coli protein purification recombinant allophycocyanin tumor inhibition |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-005-5794-7 |
| 文献子类 | Article |
| 英文摘要 | A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1). |
| WOS关键词 | STABILITY ; PLASMID |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231065100008 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273862]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Qin, S |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, BS,Tang, ZH,Lin, LP,et al. Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2005,27(11):783-787. |
| APA | Ge, BS,Tang, ZH,Lin, LP,Ren, YH,Yang, Y,&Qin, S.(2005).Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli.BIOTECHNOLOGY LETTERS,27(11),783-787. |
| MLA | Ge, BS,et al."Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli".BIOTECHNOLOGY LETTERS 27.11(2005):783-787. |
入库方式： OAI收割
来源：上海药物研究所
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