The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme
文献类型：期刊论文
| 作者 | Dong, L; Cheng, N; Wang, MW ; Zhang, JF; Shu, C; Zhu, DX
|
| 刊名 | MICROBIOLOGY-SGM
 |
| 出版日期 | 2005-06 |
| 卷号 | 151页码:2017-2023 |
| ISSN号 | 1350-0872 |
| DOI | 10.1099/mic.0.27767-0 |
| 文献子类 | Article |
| 英文摘要 | This study describes the cloning, genetic analysis and biochemical characterization of a leucyl aminopeptidase (LAP) from Helicobacter pylori. A gene encoding LAP was cloned from H. pylori and the expressed 55 kDa protein displayed homology to aminopeptidases from Gram-negative bacteria, plants and mammals. This LAP demonstrated amidolytic activity against L-leucine-p-nitroanilide. Optimal activity was observed at pH 8-0 and 45 degrees C, with V-max of 232 mu mol min(-1) (mg protein)(-1) and S-0.5 of 0.65 mM. The data suggest that LAP could be allosteric (n(H) = 2.27), with regulatory homohexamers, and its activity was inhibited by ion chelators and enhanced by divalent manganese, cobalt and nickel cations. Bestatin inhibited both LAP activity (IC50 = 49.9 nM) and H. pylori growth in vitro. The results point to the potential use of LAP as a drug target to develop novel anti-H. pylori agents. |
| WOS关键词 | INTRACELLULAR PROTEIN DEGRADATION ; LENS LEUCINE AMINOPEPTIDASE ; ESCHERICHIA-COLI ; BOVINE LENS ; SALMONELLA-TYPHIMURIUM ; BACTERIAL-CELLS ; BINDING-SITES ; PURIFICATION ; BESTATIN ; GROWTH |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | MICROBIOLOGY SOC |
| WOS记录号 | WOS:000230041200028 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273867]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhu, DX |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 210203, Peoples R China 2.Nanjing Univ, Dept Biochem, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, L,Cheng, N,Wang, MW,et al. The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme[J]. MICROBIOLOGY-SGM,2005,151:2017-2023. |
| APA | Dong, L,Cheng, N,Wang, MW,Zhang, JF,Shu, C,&Zhu, DX.(2005).The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme.MICROBIOLOGY-SGM,151,2017-2023. |
| MLA | Dong, L,et al."The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme".MICROBIOLOGY-SGM 151(2005):2017-2023. |
入库方式： OAI收割
来源：上海药物研究所
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