Determination of psoralen and isopsoralen in traditional Chinese medicines by capillary zone electrophoresis with amperometric detection
文献类型：期刊论文
| 作者 | Wu, F; Wang, AF; Zhou, Y ; Zhou, TS; Jiang, HL; Fang, YZ
|
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2005-02 |
| 卷号 | 61期号:3-4页码:157-160 |
| 关键词 | capillary electrophoresis amperometric detection psoralen isopsoralen |
| ISSN号 | 0009-5893 |
| DOI | 10.1365/s10337-004-0476-5 |
| 文献子类 | Article |
| 英文摘要 | A simple, reliable and reproducible method was developed for determination of psoralen and isopsoralen in several traditional Chinese medicines, such as Shouwu Pian, Gubenkechuan Pian, was described in this paper. It was based on capillary zone electrophoresis with amperometric detection (CZE-AD). A carbon-disc electrode was used as working electrode. The optimal conditions of separation and detection were pH7.6 phosphate buffer (20 mM), 20 kV for the separation voltage and 1.00V (vs. Ag/AgCl) for the detection potential. The linear ranges were 6.90 x 10(-5similar to)1.30 x 10(-7) mol L-1 for psoralen and 5.0 x 10(-5)similar to1.80 x 10(-7) mol.L-1 for isopsoralen with the correlation coefficient of 0.9984 and 0.9994, respectively. The detection limits for psoralen and isopsoralen were 4.2 x 10(-8) mol.L-1 and 6.0 x 10(-8) mol.L-1 based on the signal to noise ratio of 3. The method built in this paper was directly applied to the determination of several traditional Chinese medicines with some simple pretreatment. The assay result was satisfactory. |
| WOS关键词 | CHROMATOGRAPHIC DETERMINATION ; ELECTROCHEMICAL DETECTION ; PUERARIA-LOBATA ; PHOTOCHEMOTHERAPY ; SEPARATION ; RUTIN ; SKIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000227123900010 |
| 出版者 | VIEWEG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273924]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Fang, YZ |
| 作者单位 | 1.E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, F,Wang, AF,Zhou, Y,et al. Determination of psoralen and isopsoralen in traditional Chinese medicines by capillary zone electrophoresis with amperometric detection[J]. CHROMATOGRAPHIA,2005,61(3-4):157-160. |
| APA | Wu, F,Wang, AF,Zhou, Y,Zhou, TS,Jiang, HL,&Fang, YZ.(2005).Determination of psoralen and isopsoralen in traditional Chinese medicines by capillary zone electrophoresis with amperometric detection.CHROMATOGRAPHIA,61(3-4),157-160. |
| MLA | Wu, F,et al."Determination of psoralen and isopsoralen in traditional Chinese medicines by capillary zone electrophoresis with amperometric detection".CHROMATOGRAPHIA 61.3-4(2005):157-160. |
入库方式： OAI收割
来源：上海药物研究所
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