Characterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects
文献类型：期刊论文
| 作者 | Yu, MS; Lai, SW; Lin, KF; Fang, JN; Yuen, WH; Chang, RCC |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
 |
| 出版日期 | 2004-11 |
| 卷号 | 14期号:5页码:917-924 |
| ISSN号 | 1107-3756 |
| 关键词 | Nerium indicum family polysaccharide neuroprotection neurotoxicity beta-amyloid peptide traditional Chinese medicine |
| 文献子类 | Article |
| 英文摘要 | Degeneration of neurons is a key problem in Alzheimer's disease (AD) and neuroprotection is a possible way to safeguard neurons from neurodegeneration. Polysaccharides isolated from Chinese medicinal herbs have been investigated extensively for their anti-tumor and immune stimulating effects. Yet, little is known about the effects of polysaccharides in neurons. Recently, two pure polysaccharides isolated from the flowers of Nerium indicum were shown to stimulate proliferation and differentiation of PC12 pheochromocytorna cells, an effect similar to that observed from nerve growth factor. In this notion, it is hypothesized that polysaccharides isolated from the flowers of N. indicum could exhibit beneficial effects in neurons. In this study, we isolated, characterized and investigated two new polysaccharides from the flowers of N. indicum for their neuroprotective effects on neurons against serum-deprivation and beta-amyloid (Abeta) peptide toxicity in primary rat cortical neuronal cultures. Pretreatment of the polysaccharides significantly reduced the number of apoptotic neurons revealed by DAPI staining when neurons were exposed to serum-free medium. Besides, the polysaccharides could also decrease the activity of caspase-3 triggered by Abeta peptides. Western blot analysis indicated that polysaccharides stimulated the phosphorylation of PDK-1 (Serine 241) and Akt (Threonine 308). In conclusion, the polysaccharides J2, J3 and J4 isolated from N. indicum provide a lead for future development of neuroprotective agent against neuronal death in neurodegenerative diseases and the neuroprotective mechanism may primarily rely on activation of Akt survival signaling pathway. |
| WOS关键词 | EUKARYOTIC INITIATION FACTOR-2-ALPHA ; AMYLOID PEPTIDE TOXICITY ; MESENCEPHALIC CULTURES ; NEURONAL DEGENERATION ; ALZHEIMERS-DISEASE ; PROTEIN-KINASE ; CELL-DEATH ; APOPTOSIS ; PHOSPHORYLATION ; ACTIVATION |
| WOS研究方向 | Research & Experimental Medicine |
| 语种 | 英语 |
| 出版者 | SPANDIDOS PUBL LTD |
| WOS记录号 | WOS:000224737700022 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273998]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chang, RCC |
| 作者单位 | 1.Univ Hong Kong, Inst Mol Technol Drug Discovery & Synth, Open Lab Chem Biol, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.Univ Hong Kong, Fac Med, Dept Anat, Lab Neurogegenerat Dis, Hong Kong, Hong Kong, Peoples R China 4.Univ Hong Kong, Cent Lab, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, MS,Lai, SW,Lin, KF,et al. Characterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2004,14(5):917-924. |
| APA | Yu, MS,Lai, SW,Lin, KF,Fang, JN,Yuen, WH,&Chang, RCC.(2004).Characterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,14(5),917-924. |
| MLA | Yu, MS,et al."Characterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 14.5(2004):917-924. |
入库方式： OAI收割
来源：上海药物研究所
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