The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells
文献类型：期刊论文
| 作者 | Wu, XD; Shang, B; Yang, RF; Yu, H; Ma, ZH; Shen, X ; Ji, YY; Lin, Y; Wu, YD; Lin, GM
|
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2004-10 |
| 卷号 | 14期号:5页码:400-406 |
| 关键词 | SARS-CoV spike protein nucleocapsid protein envelope protein 3CL protease polyclonal antibody |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/sj.cr.7290240 |
| 文献子类 | Article |
| 英文摘要 | Spike protein is one of the major structural proteins of severe acute respiratory syndrome-coronavirus. It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection. Some spike proteins of coronavirus, such as MHV, HCoV-OC43, AIBV and BcoV, are proteolytically cleaved into two subunits, S1 and S2. In contrast, TGV, FIPV and HCoV-229E are not. Many studies have shown that the cleavage of spike protein seriously affects its function. In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S1 and S2 subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E.coli expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. Furthermore, the anti-S1 and S2 Abs were proved to be capable of binding to SARS CoV under electron microscope observation. When S2 Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2 fragment was detected with S2-specific mAb by Western blot analysis. The data demonstrated that the cleavage of S protein was observed in the lysate, indicating that proteolytic processing of S protein is present in host cells. |
| WOS关键词 | LEUKEMIA-VIRUS ; CORONAVIRUS ; SEQUENCE ; IDENTIFICATION ; GLYCOPROTEIN ; MEMBRANE ; GENOME ; GENE ; SITE |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000225478900007 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274009]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu, JR |
| 作者单位 | 1.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Inst Basic Med Sci, Beijing 100071, Peoples R China 2.Chinese Acad Sci, Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, XD,Shang, B,Yang, RF,et al. The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells[J]. CELL RESEARCH,2004,14(5):400-406. |
| APA | Wu, XD.,Shang, B.,Yang, RF.,Yu, H.,Ma, ZH.,...&Sun, B.(2004).The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells.CELL RESEARCH,14(5),400-406. |
| MLA | Wu, XD,et al."The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells".CELL RESEARCH 14.5(2004):400-406. |
入库方式： OAI收割
来源：上海药物研究所
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