The effect of Nestorone on gonadotropic cells in pituitary of rats
文献类型：期刊论文
| 作者 | Zhao, XF; Xu, YQ; Wang, YL; Leng, Y ; Cao, L; Jin, JD; Gu, ZP
|
| 刊名 | CONTRACEPTION
 |
| 出版日期 | 2004-06 |
| 卷号 | 69期号:6页码:505-511 |
| 关键词 | Nestorone LH gonadotropic cells LH beta mRNA PR mRNA |
| ISSN号 | 0010-7824 |
| DOI | 10.1016/j.contraception.2004.01.007 |
| 文献子类 | Article |
| 英文摘要 | The implant containing Nestorone is a promising long-acting contraceptive especially suitable for lactating women. In this study, two experiments were designed to observe the effect of Nestorone on the gonadotropic cells in pituitary of rats for analyzing its antiovulation mechanism. In the first experiment, the ED50 of Nestorone on inhibiting ovulation was found to be 1.32 mg/kg. The serum luteinizing hormone (LH) levels were significantly lower 60 h after being treated with Nestorone at 8:30-9:00 a.m. on Day 2 (D2) of estrus. Image analysis showed that the average size of the LH cells in groups treated with Nestorone at 2 or 4 mg/kg was larger than that of the control. In the group treated with 4 mg/kg, most of gonadotropic cells were regular round in shape. And, abundant granules in cytoplasm were found in those cells, which indicated that the LH stored in cells was not released. In the second experiment, the rats were treated with Nestorone at 5 mg/kg at 11:30-12:00 a.m. on D2 of estrus. The normal or higher expression of LHbeta mRNA in pituitary suggested that the synthesis of LH was not inhibited by the treatment with Nestorone. The expression of PR mRNA in pituitary was significantly lower than that of the control at 33 h after treatment. This might be a direct effect of Nestorone, since there were no differences in the serum E-2 and P-4 levels between the treated and the control group. It is concluded that Nestorone prevents ovulation through inhibition of LH secretion and it has no effect on synthesis of LH. Progesterone receptors in pituitary might be involved in this process, but further study is needed to gain more evidence. (C) 2004 Elsevier Inc. All rights reserved. |
| WOS关键词 | PROGESTIN ST-1435 ; IMPLANT ; ESTROGEN ; RECEPTOR |
| WOS研究方向 | Obstetrics & Gynecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000221736900012 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274069]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Gu, ZP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Zhejiang Xianju Pharmaceut Co Ltd, Xianju 317300, Peoples R China 3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, XF,Xu, YQ,Wang, YL,et al. The effect of Nestorone on gonadotropic cells in pituitary of rats[J]. CONTRACEPTION,2004,69(6):505-511. |
| APA | Zhao, XF.,Xu, YQ.,Wang, YL.,Leng, Y.,Cao, L.,...&Gu, ZP.(2004).The effect of Nestorone on gonadotropic cells in pituitary of rats.CONTRACEPTION,69(6),505-511. |
| MLA | Zhao, XF,et al."The effect of Nestorone on gonadotropic cells in pituitary of rats".CONTRACEPTION 69.6(2004):505-511. |
入库方式： OAI收割
来源：上海药物研究所
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