Injury of mouse brain mitochondria induced by cigarette smoke extract and effect of vitamin C on it in vitro
文献类型：期刊论文
| 作者 | Yang, YM; Liu, GT |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2003-09 |
| 卷号 | 16期号:3页码:256-266 |
| 关键词 | cigarette smoke extract nicotine vitamin C mitochondrial function mitochondrial structure |
| ISSN号 | 0895-3988 |
| 文献子类 | Article |
| 英文摘要 | Objective To investigate the toxicity of cigarette smoke extract (CSE) and nicotine on mouse brain mitochondria as well as the protective effect of vitamin C in vitro. Method Mouse brain mitochondria in vitro was incubated with CSE or nicotine in the absence or presence of vitamin C for 60 minutes, and the changes of mitochondrial function and structure were measured. Results CSE inhibited mitochondrial ATPase and cytochrome C oxidase activities in a dose-dependent manner. However, no significant changes in the peroxidation indices were observed when mitochondrial respiratory enzymes activity was inhibited, and protection of mitochondria from CSE-induced injury by vitamin C was not displayed in vitro. The effect of CSE on mouse brain mitochondria swelling response to calcium stimulation was dependent on calcium concentrations. CSE inhibited swelling of mitochondria at 6.5 mumol/L Ca2+, but promoted swelling response at 250 mumol/L Ca2+. Nicotine, the major component of cigarette smoke, showed no significant damage in mouse brain mitochondria in vitro. The CSE treatment induced mitochondrial inner membrane damage and vacuolization of the matrix, whereas the outer mitochondrial membrane appeared to be preserved. Conclusion The toxic effect of CSE on brain mitochondria may be due to its direct action on enzymatic activity rather than through oxygen free radical injury. Nicotine is not the responsible component for the toxicity of CSE to brain mitochondria. |
| WOS关键词 | PERMEABILITY TRANSITION ; EXPOSURE ; REGIONS ; MUSCLE ; HEART ; LIVER ; ASSAY ; CHAIN ; CELLS |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:000185814600008 |
| 出版者 | CHINESE ACAD PREVENTIVE MEDICINE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274190]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, GT |
| 作者单位 | Chinese Acad Sci, Peking Union Med Coll, Inst Mat Med, Div Pharmacol, Beijing 100050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, YM,Liu, GT. Injury of mouse brain mitochondria induced by cigarette smoke extract and effect of vitamin C on it in vitro[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2003,16(3):256-266. |
| APA | Yang, YM,&Liu, GT.(2003).Injury of mouse brain mitochondria induced by cigarette smoke extract and effect of vitamin C on it in vitro.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,16(3),256-266. |
| MLA | Yang, YM,et al."Injury of mouse brain mitochondria induced by cigarette smoke extract and effect of vitamin C on it in vitro".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 16.3(2003):256-266. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。