Nanoparticles bearing polyethyleneglycol-coupled transferrin as gene carriers: preparation and in vitro evaluation
文献类型：期刊论文
| 作者 | Li, YP ; Ogris, M; Wagner, E; Pelisek, J; Ruffer, M
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2003-06-18 |
| 卷号 | 259期号:1-2页码:93-101 |
| 关键词 | pDNA nanoparticles transferrin polyethylene glycol poly(cyanoacrylate) |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/S0378-5173(03)00211-4 |
| 文献子类 | Article |
| 英文摘要 | The aims of this work were to determine the stability of pDNA against various conditions during microencapsulation, prepare transferrin (TF)-conjugated PEGylated polycyanoacrylate nanoparticles (TF-PEG-nanoparticles), and assess its physicochemical characteristics and in vitro targeting cells association. The open circular forms of pDNA obviously increased when pDNA was emulsified into organic solution under sonification. When pDNA solution (pH 7.0) contained 1, 3 or 5% (w/v) PVA, after sonification, average 48.2, 59.4 and 62.1% of double-supercoiled DNA (dsDNA) were preserved, respectively. When medium of pDNA was 0.9% NaCl (pH 7.0), 0.1 M NaHCO3 (pH 8.0) or phosphate buffer (pH 8.0), average 53.1, 69.3 and 56.9% of dsDNA remained after sonification, respectively. Poly(aminopoly(ethylene glycol)cyanoacrylate-co-hexadecyl cyanoacrylate) (poly(H(2)NPEGCA-co-HDCA)) showed a slight influence on pDNA in 0.1 M NaHCO3 (pH 8.0) when its concentration increased from 0.5 to 4% (w/v). TF-PEG-nanoparticles loading pDNA were spherical in shape with size under 200 nm and entrapment efficiency 35-50%. 0.1 M NaHCO3 with 3% PVA (w/v) could largely reduce the damage of pDNA during microencapsulation. TF-PEG-nanoparticles bore 1-3% of the total PEG chains conjugated to TF molecules, and exhibited the burst effect with over 30% drug release within I day. After the first phase, pDNA release profiles displayed a sustained release. The amount of cumulated pDNA release over 7 days was: 86.3, 81.5 and 74.4% for 1, 2 and 4% polymer nanoparticles, respectively. The degree of target K562 cell binding of TF-PEG-nanoparticles was greater than that of non-targeted PEG-nanoparticles at 4 degreesC. The presence of free TF decreased significantly the degree of cell binding of TF-PEG-nanoparticles, which revealed that the binding of TF-PEG-nanoparticles to K562 cells was indeed receptor specific. These results suggested that TF-PEG-nanoparticles were useful for delivery of pDNA to target cells. (C) 2003 Elsevier Science B.V. All rights reserved. |
| WOS关键词 | FACTOR-ALPHA CARRIERS ; PLASMID DNA ; IN-VIVO ; POLYCYANOACRYLATE NANOPARTICLES ; DEOXYRIBONUCLEIC ACID ; PROTEIN CARRIERS ; DELIVERY ; LIPOSOMES ; RECEPTOR ; RELEASE |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000183510300009 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274218]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, YP |
| 作者单位 | 1.Univ Munich, Ctr Drug Res, Dept Pharm, D-81377 Munich, Germany 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, YP,Ogris, M,Wagner, E,et al. Nanoparticles bearing polyethyleneglycol-coupled transferrin as gene carriers: preparation and in vitro evaluation[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2003,259(1-2):93-101. |
| APA | Li, YP,Ogris, M,Wagner, E,Pelisek, J,&Ruffer, M.(2003).Nanoparticles bearing polyethyleneglycol-coupled transferrin as gene carriers: preparation and in vitro evaluation.INTERNATIONAL JOURNAL OF PHARMACEUTICS,259(1-2),93-101. |
| MLA | Li, YP,et al."Nanoparticles bearing polyethyleneglycol-coupled transferrin as gene carriers: preparation and in vitro evaluation".INTERNATIONAL JOURNAL OF PHARMACEUTICS 259.1-2(2003):93-101. |
入库方式： OAI收割
来源：上海药物研究所
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