Identification of an epitope of SARS-coronavirus nucleocapsid protein
文献类型：期刊论文
| 作者 | Lin, Y; Shen, X ; Yang, RF; Li, YX; Ji, YY; He, YY; Shi, MD; Lu, W; Shi, TL; Wang, J
|
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2003-06 |
| 卷号 | 13期号:3页码:141-145 |
| 关键词 | severe acute respiratory syndrome-coronavirus necleocapsid protein epitope polyclonal antibody antiserum |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/sj.cr.7290158 |
| 文献子类 | Article |
| 英文摘要 | The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, it was confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-Cov. |
| WOS关键词 | INFECTIOUS-BRONCHITIS VIRUS |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000183929800001 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274228]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu, JR |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Inst Mat Med,Bioinformat Ctr, Shanghai 200031, Peoples R China 2.Acad Mil Med Sci, Inst Basic Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Y,Shen, X,Yang, RF,et al. Identification of an epitope of SARS-coronavirus nucleocapsid protein[J]. CELL RESEARCH,2003,13(3):141-145. |
| APA | Lin, Y.,Shen, X.,Yang, RF.,Li, YX.,Ji, YY.,...&Sun, B.(2003).Identification of an epitope of SARS-coronavirus nucleocapsid protein.CELL RESEARCH,13(3),141-145. |
| MLA | Lin, Y,et al."Identification of an epitope of SARS-coronavirus nucleocapsid protein".CELL RESEARCH 13.3(2003):141-145. |
入库方式： OAI收割
来源：上海药物研究所
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