C-Myc degradation induced by DNA damage results in apoptosis of CHO cells
文献类型：期刊论文
| 作者 | Jiang, MR; Lie, YC; Yang, Y ; Wu, JR
|
| 刊名 | ONCOGENE
 |
| 出版日期 | 2003-05-22 |
| 卷号 | 22期号:21页码:3252-3259 |
| 关键词 | c-Myc apoptosis DNA damage CHO cell tripchlorolide |
| ISSN号 | 0950-9232 |
| DOI | 10.1038/sj.onc.1206501 |
| 文献子类 | Article |
| 英文摘要 | Although tripchlorolide (TC), a compound purified from a Chinese herb Tripterygium Wilfordii Hook, has been demonstrated to be a potent antitumor agent, its mechanisms of action are unknown. The present study shows that TC induces apoptosis of Chinese Hamster Ovary (CHO) cells. Most strikingly, TC was particularly potent in inducing apoptosis of the UV41 mutant CHO cells, which are deficient in the ERCC4 gene encoding a nucleotide excision repair protein. TC caused a higher level of DNA damage in UV41 cells than those in the wildtype CHO cells or EM9 cells, which are deficient in single-strand break repair. These results provided a critical link between apoptotic hypersensitivity and DNA damage in defective nucleotide excision repair pathway of UV41 cells by TC treatment. Further analysis showed that degradation of the c-Myc protein in TC-treated UV41 cells was much stronger than those in the wild-type CHOAA8 and the EM9. A proteasome inhibitor, MG132, reduced both the degradation of c-Myc and apoptosis in TC-treated UV41 cells. Expression of exogenous c-Myc also inhibited apoptosis of TC-treated UV41 cells. These results indicate that c-Myc degradation induced by DNA damage in the presence of TC contributes to induction of apoptosis of UV41 cells. |
| WOS关键词 | SISTER-CHROMATID EXCHANGE ; STRAND-BREAK REPAIR ; PROTEIN-DEGRADATION ; TRIPTOLIDE ; PROTEASOME ; EXPRESSION ; GROWTH ; CHEMOTHERAPY ; SUPPRESSION ; INHIBITORS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000183040000007 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274235]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu, JR |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, MR,Lie, YC,Yang, Y,et al. C-Myc degradation induced by DNA damage results in apoptosis of CHO cells[J]. ONCOGENE,2003,22(21):3252-3259. |
| APA | Jiang, MR,Lie, YC,Yang, Y,&Wu, JR.(2003).C-Myc degradation induced by DNA damage results in apoptosis of CHO cells.ONCOGENE,22(21),3252-3259. |
| MLA | Jiang, MR,et al."C-Myc degradation induced by DNA damage results in apoptosis of CHO cells".ONCOGENE 22.21(2003):3252-3259. |
入库方式： OAI收割
来源：上海药物研究所
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