Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia
文献类型：期刊论文
| 作者 | Wang, RG; Hu, XZ |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2002-05 |
| 卷号 | 23期号:5页码:405-411 |
| 关键词 | endothelial growth factors angiopoietin-1 angiopoietin-2 Tie-1 Tie-2 inbred C57BL mice brain ischemia reverse transcriptase polymerase chain reaction messenger RNA immunohistochemistry |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the expressions of vascular endothelial growth factor ( VEGF), angiopoietin-1 ( Ang-1), angiopoietin-2 (Ang-2), Tie-1, and Tie-2 in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS: The mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were measured by semiquantitative reverse transcription polymerase chain reaction ( RTPCR). The protein expressions of VEGF and Ang-2 were determined by immunohistochemistry. RESULTS: Low mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were constitutively expressed in the normal cortex of mouse. After middle cerebral artery occlusion (MCAO), the expressions of VEGF, Ang-2, and Tie-2 mRNA were dramatically increased in the infarcted cortex and the elevation was remained through 7 d of ischemia. However, the levels of Ang-1 and Tie-1 mRNA were unchanged in the infarcted cortex. Immunoreactivities of Ang-2 or VEGF were hardly observed in the normal cortex. Ang-2 protein was evidently detected in the infarct core 8 h after MCAO and in the perifocal area I d after MCAO. Expression of VEGF protein was elevated in the infarct core 2 h after MCAO and in the perifocal area I d after MCAO. Immunoreaction was restricted to endothelial cells and glial-like cells within the infarct core and perifocal area. CONCLUSION: The expressions of Ang-2 and VEGF are induced after focal cerebral ischemia, which may contribute to the angiogenic response in the cortex of ischemic brain. |
| WOS关键词 | ARTERY OCCLUSION ; ANGIOGENESIS ; INDUCTION ; RECEPTOR ; VEGF ; TIE2 ; RAT |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1366691 |
| WOS记录号 | WOS:000175399400005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274363]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, RG |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, RG,Hu, XZ. Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia[J]. ACTA PHARMACOLOGICA SINICA,2002,23(5):405-411. |
| APA | Wang, RG,&Hu, XZ.(2002).Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia.ACTA PHARMACOLOGICA SINICA,23(5),405-411. |
| MLA | Wang, RG,et al."Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia".ACTA PHARMACOLOGICA SINICA 23.5(2002):405-411. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。