Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine
文献类型：期刊论文
| 作者 | Guan, XJ; Wu, YZ; Jia, ZC; Shi, TD; Tang, Y |
| 刊名 | WORLD JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2002-04 |
| 卷号 | 8期号:2页码:294-297 |
| ISSN号 | 1007-9327 |
| 文献子类 | Article |
| 英文摘要 | AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes; of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes ( ISCOMS I based vaccine containing a novel therapeutic hepatits B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40 nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33 g(.)L(-1). At the paralleled position close to the sixth band of the molecular weight marker (3480 kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of EUSPOT assay, which showed that lFN-gamma producing cells ( specific CTL responses) were increased (spots of ISCOMS-treated group: 47 +/- 5. n = 3; control group: 5 +/- 2, n = 3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo. |
| WOS关键词 | T-CELL RESPONSES ; IMMUNE-RESPONSES ; INFLUENZA-VIRUS ; RHESUS MACAQUES ; IN-VIVO ; HBV DNA ; EXPRESSION ; PROTECTION ; ACTIVATION ; INFECTION |
| WOS研究方向 | Gastroenterology & Hepatology |
| 语种 | 英语 |
| WOS记录号 | WOS:000175104600021 |
| 出版者 | BAISHIDENG PUBL GRP CO LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274374]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu, YZ |
| 作者单位 | 1.Third Mil Med Univ, Inst Immunol, Chongqing 400038, Peoples R China 2.Second Mil Med Univ, Dept Sci & Res, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, XJ,Wu, YZ,Jia, ZC,et al. Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(2):294-297. |
| APA | Guan, XJ,Wu, YZ,Jia, ZC,Shi, TD,&Tang, Y.(2002).Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine.WORLD JOURNAL OF GASTROENTEROLOGY,8(2),294-297. |
| MLA | Guan, XJ,et al."Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine".WORLD JOURNAL OF GASTROENTEROLOGY 8.2(2002):294-297. |
入库方式： OAI收割
来源：上海药物研究所
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