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Liquid chromatography/tandem mass spectrometry for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma with deuterated fluoxetine as internal standard

文献类型：期刊论文

作者Li, C; Ji, ZH; Nan, FJ; Shao, QX; Liu, P; Dai, JY; Zhen, J; Yuan, H; Xu, F; Cui, J
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
出版日期2002
卷号16期号:19页码:1844-1850
ISSN号0951-4198
DOI10.1002/rcm.800
文献子类Article
英文摘要Fluoxetine (F) and its N-demehylated metabolite norfluoxetine (NF) are selective inhibitors of serotonin reuptake in humans. A new sensitive rapid method for the simultaneous determination of F and NF in plasma was established and validated, and was further applied to assess the bioequivalence of two oral formulations of F in 22 healthy Chinese male volunteers who received a single oral dose of each formulation (containing 20 mg of fluoxetine hydrochloride). The new method involves using liquid chromatography/tandem mass spectrometry (LC/MS/MS) in multiple reaction monitoring mode with deuterated fluoxetine (DF) as internal standard. High levels of analytical sensitivity and specificity of MS/MS detection enabled use of a simple liquid-liquid extraction procedure. The combination of a simple sample clean-up procedure and short chromatographic run-time (5 min) considerably increased the productivity of the analytical method. The method was validated for the plasma concentration range 0.27-22 ng/mL for both of the test compounds, and the calibration curves were linear with coefficients of correlation >0.999. The limit of detection was 0.1 ng/mL for plasma F and NF. Taking the plasma sample size (200 muL) into account the new method for determination of F and NF is more sensitive than those described previously. Copyright (C) 2002 John Wiley Sons, Ltd.
WOS关键词SEROTONIN REUPTAKE INHIBITORS ; ELECTRON-CAPTURE DETECTION ; GAS-CHROMATOGRAPHY ; FLUORESCENCE DETECTION ; ULTRAVIOLET DETECTION ; ENANTIOMERS ; ANTIDEPRESSANTS ; QUANTIFICATION ; SAMPLES ; SERUM
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
WOS记录号WOS:000178363600007
出版者JOHN WILEY & SONS LTD
源URL[http://119.78.100.183/handle/2S10ELR8/274413]  
专题中国科学院上海药物研究所
通讯作者Li, C
作者单位1.Eli Lilly Asia Inc, Shanghai 200001, Peoples R China
2.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, SIBS, Shanghai 200031, Peoples R China
4.Zhejiang Acad Med Sci, Inst Clin Pharmacol, Hangzhou 310013, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Li, C,Ji, ZH,Nan, FJ,et al. Liquid chromatography/tandem mass spectrometry for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma with deuterated fluoxetine as internal standard[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2002,16(19):1844-1850.
APA Li, C.,Ji, ZH.,Nan, FJ.,Shao, QX.,Liu, P.,...&Yu, C.(2002).Liquid chromatography/tandem mass spectrometry for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma with deuterated fluoxetine as internal standard.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,16(19),1844-1850.
MLA Li, C,et al."Liquid chromatography/tandem mass spectrometry for the determination of fluoxetine and its main active metabolite norfluoxetine in human plasma with deuterated fluoxetine as internal standard".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 16.19(2002):1844-1850.

入库方式： OAI收割

来源：上海药物研究所

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