PEGylated polycyanoacrylate nanoparticles as tumor necrosis factor-alpha carriers
文献类型：期刊论文
| 作者 | Li, YP ; Pei, YY; Zhou, ZH; Zhang, XY; Gu, ZH; Ding, J; Zhou, JJ; Gao, XJ
|
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2001-04-28 |
| 卷号 | 71期号:3页码:287-296 |
| 关键词 | poly(ethylene glycol) poly(alkyl cyanoacrylate) nanoparticles tumor necrosis factor-alpha recombinant human tumor necrosis factor-alpha poly(methoxy-polyethyleneglycol cyanoacrylate-co-n-hexadecy cyanoacrylate) |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/S0168-3659(01)00235-8 |
| 文献子类 | Article |
| 英文摘要 | The aim of this study was to find an effective carrier for recombinant human tumor necrosis factor-alpha (rHuTNF-alpha). The influence of solvent systems containing poly(methoxy-polyethyleneglycol cyanoacrylate-co-n-hexadecyl cyanoacrylate) (PEGylated PHDCA) on the biological activity of rHuTNF-alpha was investigated. The PEGylated PHDCA nanoparticles loading rHuTNF-alpha were prepared with the double emulsion method. The influence of main experimental factors on the entrapment efficiency was evaluated hy the Uniform Design. The physicochemical characteristics and in vitro release of rHuTNF-alpha from the nanoparticles were determined. The results showed that serum albumin such as human serum albumin (HSA) or bovine serum albumin (BSA) could play a protective action on rHuTNF-alpha in the preparation process. At greater than or equal to2.0% (w/v) HSA concentration, mon than 85% of rHuTNF-alpha activity remained and the role of HSA was not affected by copolymer concentrations from 0.5 to 3.0% (w/v). The entrapment efficiency of the nanoparticles was about 60% and the nanoparticle size was about 150 nm. The nanoparticles were spherical in shape and uniform with the value of the zeta potential about -9 mV. The rHuTNF-alpha release from the nanoparticle showed an initial burst and then continued in a sustained fashion. The results showed that the PEGylated PHDCA nanoparticles could be an effective carrier for rHuTNF-alpha. (C) 2001 Elsevier Science B.V. All rights reserved. |
| WOS关键词 | TNF-ALPHA ; MICROSPHERES ; NANOSPHERES ; INTERFERON ; LIPOSOMES ; DELIVERY ; SARCOMA ; SYSTEM ; RATS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000168289000007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274500]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, YP |
| 作者单位 | 1.Fudan Univ, Sch Pharm, Dept Pharmaceut, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, YP,Pei, YY,Zhou, ZH,et al. PEGylated polycyanoacrylate nanoparticles as tumor necrosis factor-alpha carriers[J]. JOURNAL OF CONTROLLED RELEASE,2001,71(3):287-296. |
| APA | Li, YP.,Pei, YY.,Zhou, ZH.,Zhang, XY.,Gu, ZH.,...&Gao, XJ.(2001).PEGylated polycyanoacrylate nanoparticles as tumor necrosis factor-alpha carriers.JOURNAL OF CONTROLLED RELEASE,71(3),287-296. |
| MLA | Li, YP,et al."PEGylated polycyanoacrylate nanoparticles as tumor necrosis factor-alpha carriers".JOURNAL OF CONTROLLED RELEASE 71.3(2001):287-296. |
入库方式： OAI收割
来源：上海药物研究所
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