中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis

文献类型:期刊论文

作者Ma, XQ; Zhu, DY; Li, SP; Dong, TTX; Tsim, KWK
刊名PLANTA MEDICA
出版日期2001-03
卷号67期号:2页码:183-186
ISSN号0032-0943
DOI10.1055/s-2001-11533
文献子类Article
英文摘要Stigma Croci, stigma of Crocus sativus L., is a precious traditional Chinese medicine, which is commonly used to activate blood circulation and to dissipate blood stasis. Three plant species, Carthamus tinctorius L., Hemerocallis fulva (L.) L. and Hemerocallis citrina Baroni, could carry the name Stigma Croci in the commercial markets of South East Asia. However, C.sativus is the only one that has proven its effectiveness, while the others could act as adulterants. The authentic identification of C. sativus on the market is difficult. By using molecular genetic method, the spacer domains of 5S-rRNA were cloned from the genomic DNAs that were isolated from C.sativus, C. tinctorius, H. fulva and H. citrina. The cDNAs encoding the spacer domains, about 300 to 500 bp, were sequenced. The nucleotide sequences of these four species showed great diversity, which could serve as markers for authentic identification of Stigma Croci to distinguish from its substitution and counterfeit.
WOS关键词5S-RIBOSOMAL-RNA
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
WOS记录号WOS:000167827400024
出版者GEORG THIEME VERLAG KG
源URL[http://119.78.100.183/handle/2S10ELR8/274520]  
专题中国科学院上海药物研究所
通讯作者Tsim, KWK
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Shanghai 200031, Peoples R China
2.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China
3.Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Hong Kong, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Ma, XQ,Zhu, DY,Li, SP,et al. Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis[J]. PLANTA MEDICA,2001,67(2):183-186.
APA Ma, XQ,Zhu, DY,Li, SP,Dong, TTX,&Tsim, KWK.(2001).Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis.PLANTA MEDICA,67(2),183-186.
MLA Ma, XQ,et al."Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis".PLANTA MEDICA 67.2(2001):183-186.

入库方式: OAI收割

来源:上海药物研究所

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