Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis
文献类型:期刊论文
作者 | Ma, XQ; Zhu, DY; Li, SP; Dong, TTX; Tsim, KWK |
刊名 | PLANTA MEDICA
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出版日期 | 2001-03 |
卷号 | 67期号:2页码:183-186 |
ISSN号 | 0032-0943 |
DOI | 10.1055/s-2001-11533 |
文献子类 | Article |
英文摘要 | Stigma Croci, stigma of Crocus sativus L., is a precious traditional Chinese medicine, which is commonly used to activate blood circulation and to dissipate blood stasis. Three plant species, Carthamus tinctorius L., Hemerocallis fulva (L.) L. and Hemerocallis citrina Baroni, could carry the name Stigma Croci in the commercial markets of South East Asia. However, C.sativus is the only one that has proven its effectiveness, while the others could act as adulterants. The authentic identification of C. sativus on the market is difficult. By using molecular genetic method, the spacer domains of 5S-rRNA were cloned from the genomic DNAs that were isolated from C.sativus, C. tinctorius, H. fulva and H. citrina. The cDNAs encoding the spacer domains, about 300 to 500 bp, were sequenced. The nucleotide sequences of these four species showed great diversity, which could serve as markers for authentic identification of Stigma Croci to distinguish from its substitution and counterfeit. |
WOS关键词 | 5S-RIBOSOMAL-RNA |
WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
语种 | 英语 |
WOS记录号 | WOS:000167827400024 |
出版者 | GEORG THIEME VERLAG KG |
源URL | [http://119.78.100.183/handle/2S10ELR8/274520] ![]() |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Tsim, KWK |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Shanghai 200031, Peoples R China 2.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China 3.Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Hong Kong, Hong Kong, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, XQ,Zhu, DY,Li, SP,et al. Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis[J]. PLANTA MEDICA,2001,67(2):183-186. |
APA | Ma, XQ,Zhu, DY,Li, SP,Dong, TTX,&Tsim, KWK.(2001).Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis.PLANTA MEDICA,67(2),183-186. |
MLA | Ma, XQ,et al."Authentic identification of stigma croci (Stigma of Crocus sativus) from its adulterants by molecular genetic analysis".PLANTA MEDICA 67.2(2001):183-186. |
入库方式: OAI收割
来源:上海药物研究所
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