Inhibitory effect of nomegestrol acetate on steroidogenesis of cultured granulosa cells from rat ovary in vitro
文献类型：期刊论文
| 作者 | Qian, LH; Yang, B; Leng, Y ; Cao, L; Gu, ZP
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001-01 |
| 卷号 | 22期号:1页码:40-44 |
| 关键词 | estrogen progesterone cultured cells nomegestrol ovary |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the effect of nomegestrol acetate, a new synthetic progesterone on granulosa cells' viability and steroidogenesis function. METHODS: Granulosa cells were cultured in McCoy's 5A medium. Trypan blue stain was used to measure viable cells. FSH and testosterone were added to stimulate the steroid secretion. Specific RIA assay was used to evaluate the estrogen and progesterone secretion respectively. RESULTS:IC50 of nomegestrol acetate to damage cells is 6.85 mg/L, (95 % confidence limits 5.36-8.75 mg/L). Nomegestrol acetate 0.45, 0.9, and 1.8 mg/L, greatly inhibited the estrogen secretion from granulosa cells by 7.6 %, 12.5 %, 28.3 % in the presence of testosterone 0.5 mu mol/L and FSH 10 U/L without affecting the number of viable cells. The secretion of progesteron were markedly decreased by 44.5 %, 53.3 %, and 62.0 % concurrently. CONCLUSION: Nomegestrol acetate directly inhibited the steroidogenesis of granulosa cells. |
| WOS关键词 | FOLLICLE-STIMULATING-HORMONE ; SUBDERMAL IMPLANT ; CARBOHYDRATE-METABOLISM ; CERVICAL-MUCUS ; UNIPLANT ; WOMEN ; RECEPTORS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:515755 |
| WOS记录号 | WOS:000166435900009 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274531]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Cao, L |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, LH,Yang, B,Leng, Y,et al. Inhibitory effect of nomegestrol acetate on steroidogenesis of cultured granulosa cells from rat ovary in vitro[J]. ACTA PHARMACOLOGICA SINICA,2001,22(1):40-44. |
| APA | Qian, LH,Yang, B,Leng, Y,Cao, L,&Gu, ZP.(2001).Inhibitory effect of nomegestrol acetate on steroidogenesis of cultured granulosa cells from rat ovary in vitro.ACTA PHARMACOLOGICA SINICA,22(1),40-44. |
| MLA | Qian, LH,et al."Inhibitory effect of nomegestrol acetate on steroidogenesis of cultured granulosa cells from rat ovary in vitro".ACTA PHARMACOLOGICA SINICA 22.1(2001):40-44. |
入库方式： OAI收割
来源：上海药物研究所
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