Sequence variations of the hypervariable region of hepatitis C virus and their clinical significance
文献类型：期刊论文
| 作者 | Zhang, SC; Wang, W; Zhou, K; Liu, HY |
| 刊名 | CHINESE MEDICAL JOURNAL
 |
| 出版日期 | 2000-12 |
| 卷号 | 113期号:12页码:1075-1079 |
| 关键词 | hepatitis C hepatitis C virus hypervariable region sequence analysis reverse transcription-polymerase chain reaction type IV collagen |
| ISSN号 | 0366-6999 |
| 文献子类 | Article |
| 英文摘要 | Objective To understand the clinical significance of sequence variations in the hypervariable region (HVR) of hepatitis C virus during infection. Methods Eight patients with acute hepatitis C and 20 patients with chronic hepatitis C were followed up for two years. Blood samples were taken at intervals of six months for analysis of HCV-HVR sequences by reverse transcription-polymerase chain reaction (RT-PCR) and direct sequencing methods. Results HCV-HVR sequences of the 28 patients changed in various degrees. 92% of these nucleotide substitutions led to changes of corresponding amino- acid sequences. Only 8% of changed nucleotide were synonymous substitutions. Of 27 amino acids variation of amino acid ranged from 1 to 20 (mean 8, 30%). The most common nucleotide substitution (62%) occurred in the first position of codon, 31% in the second and the rest in the third. HVR variation rate was 0.89 x 10(-1) per genome site per year in acute hepatitis C, compared with 2.31 x 10(-1) per genome site per year in chronic hepatitis C (P < 0.05), but had no relation to HCV subtype. Variation of HVR in the flare up type (ALT > 150 mu /L) was much more than that in the quiescent type (ALT < 100 |
| WOS关键词 | HUMORAL IMMUNE-RESPONSE ; STRAND CONFORMATION POLYMORPHISM ; NON-B-HEPATITIS ; INTERFERON TREATMENT ; NON-A ; INFECTION ; ANTIBODY ; TRANSFUSION ; GENOTYPES ; PROTEINS |
| WOS研究方向 | General & Internal Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000165752700005 |
| 出版者 | CHINESE MEDICAL ASSOCIATION |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274553]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhang, SC |
| 作者单位 | 1.Shanghai Med Univ, Zhongshan Hosp, Dept Gastroenterol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Inst Mat Med, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, SC,Wang, W,Zhou, K,et al. Sequence variations of the hypervariable region of hepatitis C virus and their clinical significance[J]. CHINESE MEDICAL JOURNAL,2000,113(12):1075-1079. |
| APA | Zhang, SC,Wang, W,Zhou, K,&Liu, HY.(2000).Sequence variations of the hypervariable region of hepatitis C virus and their clinical significance.CHINESE MEDICAL JOURNAL,113(12),1075-1079. |
| MLA | Zhang, SC,et al."Sequence variations of the hypervariable region of hepatitis C virus and their clinical significance".CHINESE MEDICAL JOURNAL 113.12(2000):1075-1079. |
入库方式： OAI收割
来源：上海药物研究所
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