Comparison of the catalytic domains of collagenase-1 and stromelysin-1
文献类型：期刊论文
| 作者 | Hu, LY; Tian, SM; Ye, QZ; Ruan, KC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2000-07 |
| 卷号 | 32期号:4页码:409-412 |
| 关键词 | matrix metalloproteinase collagenase-1 stromelysin-1 high pressure ANS |
| ISSN号 | 0582-9879 |
| 文献子类 | Article |
| 英文摘要 | The catalytic domains of two matrix metalloproteinases-collagenase-1 and stromelysin-1 have been studied by means of fluorescence spectroscopy and high hydrostatic pressure. The hydrophobic fluorescence probe ANS could bind to stromelysin-1, with a dissociation constant of 26.3 mu mol/L, but could not bind to collagenase-1, indicating that there exists a hydrophobic site on the surface of stromelysin-1. Further study suggests that the hydrophobic site may not be the catalytic site. The biological activity of catalytic domains of collagenase-1 and stromelysin-1 showed obvious difference under high pressure: the activity of collagenase-1 increased with elevating pressure, with an apparent activation volume of -18.9 ml/mol; however, the activity of stromelysin-1 did not change under high pressure. The results indicate that there are some obvious differences between the catalytic domain conformations of these two enzymes, though the crystal analysis indicated that they were very similar as reported before. |
| WOS关键词 | HIGH HYDROSTATIC-PRESSURE ; MATRIX METALLOPROTEINASES ; INHIBITOR ; COMPLEX |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 中文 |
| CSCD记录号 | CSCD:737900 |
| WOS记录号 | WOS:000088319400021 |
| 出版者 | SHANGHAI INST BIOCHEMISTRY, ACADEMIA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274601]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, LY,Tian, SM,Ye, QZ,et al. Comparison of the catalytic domains of collagenase-1 and stromelysin-1[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2000,32(4):409-412. |
| APA | Hu, LY,Tian, SM,Ye, QZ,&Ruan, KC.(2000).Comparison of the catalytic domains of collagenase-1 and stromelysin-1.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,32(4),409-412. |
| MLA | Hu, LY,et al."Comparison of the catalytic domains of collagenase-1 and stromelysin-1".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 32.4(2000):409-412. |
入库方式： OAI收割
来源：上海药物研究所
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