Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain
文献类型：期刊论文
| 作者 | Ma, XQ; Duan, JA; Zhu, DY; Dong, TTX; Tsim, KWK |
| 刊名 | PHYTOCHEMISTRY
 |
| 出版日期 | 2000-06 |
| 卷号 | 54期号:4页码:363-368 |
| ISSN号 | 0031-9422 |
| 关键词 | Astragalus Fabaceae DNA sequence species identification |
| DOI | 10.1016/S0031-9422(00)00111-4 |
| 文献子类 | Article |
| 英文摘要 | About 300 species and varieties of Astragalus are identified in China, making the identification of the origin of a particular Astragalus species on the consumer market difficult. A molecular genetic approach was developed to identify various species of Astragalus. Although the 5S-rRNA coding sequence is conserved in higher eukaryotes, the spacer domain of the 5S-rRNA gene has great diversity among different species. The 5S-rRNA spacer domain was amplified by polymerase chain reaction (PCR) from the isolated genomic DNA, and the PCR products (similar to 300 bp) covering the 5S-rRNA spacer domain were sequenced. The nucleotide sequences of Astragalus membranaceus, A. membranaceus var. mongholicus, A. lehmannianus, A. hoantchy, and of one closely related species Hedysarum polybotrys (Hongqi), were determined. Diversity in DNA sequence and restriction enzyme mapping among various species was found in their 5S-rRNA spacer domains. This is the first report on the detection of 5S-rRNA spacer region sequence of Astragalus, and the results could be used for genetic identification of Huangqi. (C) 2000 Elsevier Science Ltd. All rights reserved. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000087957900003 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274619]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Tsim, KWK |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Shanghai 200031, Peoples R China 3.Jiangsu Res Inst Tradit Chinese Med, Nanjing 210028, Peoples R China 4.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, XQ,Duan, JA,Zhu, DY,et al. Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain[J]. PHYTOCHEMISTRY,2000,54(4):363-368. |
| APA | Ma, XQ,Duan, JA,Zhu, DY,Dong, TTX,&Tsim, KWK.(2000).Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain.PHYTOCHEMISTRY,54(4),363-368. |
| MLA | Ma, XQ,et al."Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain".PHYTOCHEMISTRY 54.4(2000):363-368. |
入库方式： OAI收割
来源：上海药物研究所
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