Effect of Magnesium tanshinoate B on the production of nitric oxide in endothelial cells
文献类型：期刊论文
| 作者 | O, K; Cheung, F; Sung, FL; Zhu, DY; Siow, YL |
| 刊名 | MOLECULAR AND CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2000-04 |
| 卷号 | 207期号:1-2页码:35-39 |
| 关键词 | nitric oxide endothelial cells Salviae miltiorrhizae magnesium tanshinoate B |
| ISSN号 | 0300-8177 |
| DOI | 10.1023/A:1007081911734 |
| 文献子类 | Article; Proceedings Paper |
| 英文摘要 | Nitric oxide (NO) is a potent vasodilator which plays an important role in regulating vascular tones. Danshen, a Chinese herbal medicine has been widely used for the treatment of cardiovascular diseases. The objective of this study was to investigate the effect of magnesium tanshinoate B (MTB), a compound purified from Danshen, on the production of NO in human endothelial cell line (ECV304). After cells were incubated with MTB (1-10 mu M) for 1 or 4 h, amounts of NO metabolites released by cells were quantified and cellular NOS activities were determined following the conversion of [H-3]arginine to [H-3]citrulline. The NOS protein expression was determined by Western immunoblotting analysis. MTB (1-10 mu M) stimulated the release of NO and its metabolites from endothelial cells. Following MTB treatment, the cellular NOS activities were significantly enhanced with a concomitant increase in the levels of constitutive NOS (cNOS) protein mass (110-178%). Selective activation of cNOS by MTB may be employed therapeutically in modulating NO production in endothelial cells. |
| WOS关键词 | MOLECULAR-CLONING ; MOUSE MACROPHAGES ; SELECTIVE-INHIBITION ; SYNTHASE ; BIOSYNTHESIS ; ACTIVATION |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000087113200006 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274630]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Siow, YL |
| 作者单位 | 1.Univ Hong Kong, Fac Med, Dept Pharmacol, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | O, K,Cheung, F,Sung, FL,et al. Effect of Magnesium tanshinoate B on the production of nitric oxide in endothelial cells[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2000,207(1-2):35-39. |
| APA | O, K,Cheung, F,Sung, FL,Zhu, DY,&Siow, YL.(2000).Effect of Magnesium tanshinoate B on the production of nitric oxide in endothelial cells.MOLECULAR AND CELLULAR BIOCHEMISTRY,207(1-2),35-39. |
| MLA | O, K,et al."Effect of Magnesium tanshinoate B on the production of nitric oxide in endothelial cells".MOLECULAR AND CELLULAR BIOCHEMISTRY 207.1-2(2000):35-39. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。