Luteolytic effects of DL111-IT in pregnant rats
文献类型：期刊论文
| 作者 | Yang, B; Cao, L; Fang, RY; Gu, ZP |
| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 1999-09-10 |
| 卷号 | 380期号:2-3页码:145-152 |
| 关键词 | DL111-IT luteal cell apoptosis luteolysis 3 beta-HSD (3 beta-hydroxysteroid dehydrogenase) 20 alpha-HSD (20 alpha-hydroxysteroid dehydrogenase) |
| ISSN号 | 0014-2999 |
| DOI | 10.1016/S0014-2999(99)00433-1 |
| 文献子类 | Article |
| 英文摘要 | The present studies were conducted to evaluate the effects of DL111-IT [3-(2-ethyl phenyl)-5-(3-methoxy phenyl)-1H-1, 2, 4 triazol] on ovaries of pregnant rats. Pregnant rats were i.m. treated with DL111-IT 2.5 mg kg(-1) day(-1) or camellia oleum (vehicle control) 0.2 ml/day from day 6 of pregnancy for 1, 3 or 5 days. Blood and ovaries were collected 24 h after the last injection. Ovarian fresh weight and protein contents, activities of the 3 beta-hydroxysteroid dehydrogenase (SP-HSD) and 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) in ovaries, and cell apoptosis of corpus luteum (including hematoxylin-eosine stain, in situ 3'-end labeling and nucleosomal banding) were estimated. Compared with that in the control group, ovarian fresh weight declined 11% and 22% after DL111-IT-3 days and -5 days; protein content dropped 29% after 5-day administration. DL111-IT for 3 days provoked a marked decrease of serum progesterone, by 31% of the control; the activity of 3 beta-HSD decreased 34.4% after i.m. DL111-IT for 5 days, while that of 20 alpha-HSD increased dramatically after only one injection of DL111-IT (P < 0.01). Histological analysis and in situ 3'-end DNA labeling indicated that DL111-IT induced the pyknosis of cells and the formations of apoptotic bodies and intense oligonucleosomes in luteal cells of pregnant rats. The cell apoptosis induced by DL111-IT was further confirmed by evaluation of nucleosomal DNA fragmentation by agarose gel electrophoresis in cultured luteal cells exposed to DL111-IT for 24 h. In conclusion, all results, including shrunken luteal cells, decreased concentration of protein content and serum progesterone, changed activities of 3 beta-HSD and 20 alpha-HSD and formation of DNA fragments in luteal cells, showed the luteolytic effect of DL111-IT in pregnant rats. (C) 1999 Elsevier Science B.V. All rights reserved. |
| WOS关键词 | OVINE LUTEAL CELLS ; PROTEIN-KINASE-C ; CORPORA-LUTEA ; ESTROUS-CYCLE ; CORPUS-LUTEUM ; APOPTOSIS ; DEATH ; PROLIFERATION ; DEHYDROGENASE ; REGRESSION |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000082881700012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274695]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Gu, ZP |
| 作者单位 | 1.Zhejiang Univ, Sch Pharm, Dept Pharmacol, Hangzhou 310031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, B,Cao, L,Fang, RY,et al. Luteolytic effects of DL111-IT in pregnant rats[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,1999,380(2-3):145-152. |
| APA | Yang, B,Cao, L,Fang, RY,&Gu, ZP.(1999).Luteolytic effects of DL111-IT in pregnant rats.EUROPEAN JOURNAL OF PHARMACOLOGY,380(2-3),145-152. |
| MLA | Yang, B,et al."Luteolytic effects of DL111-IT in pregnant rats".EUROPEAN JOURNAL OF PHARMACOLOGY 380.2-3(1999):145-152. |
入库方式： OAI收割
来源：上海药物研究所
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