Human mu opioid receptor expressed in Sf9 insect cells was functionally coupled to endogenous G protein
文献类型：期刊论文
| 作者 | Wei, Q; Wang, TL; Chen, J ; Zhou, DH; Chi, ZQ; Shen, QX
|
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 1999-09 |
| 卷号 | 31期号:5页码:598-600 |
| 关键词 | human mu opioid receptor(H mu OR) endogenous G proteins insect cells Sf9 |
| ISSN号 | 0582-9879 |
| 文献子类 | Article |
| 英文摘要 | Human mu opioid receptor(H mu OR) with a tag of six consecutive histidines at its carboxyl terminus overexpressed in recombinant baculovirus infected Sf9 insect cells was demonstrated to be functionally coupled to endogenous G proteins. Na+ and GTP could reduce the affinity binding of etorphine and Ohmefentanyl(Ohm) to H mu OR overexpressed in Sf9. The binding of [S-35]GTP gamma S to Sf9 cell membranes containing H mu OR was stimulated by DAGO and Ohm. This stimulation could be blocked by naloxone or the pretreatment of PTX. Also, DAGO and Ohm could inhibit the increasing of intracellular cAMP stimulated by forskolin. The results showed H mu OR expressed in Sf9 insect cells were functionally coupled to endogenous PTX sensitive G(i/o) proteins. |
| WOS关键词 | DESENSITIZATION ; ACTIVATION ; AGONIST |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 中文 |
| CSCD记录号 | CSCD:737791 |
| WOS记录号 | WOS:000082673900024 |
| 出版者 | SHANGHAI INST BIOCHEMISTRY, ACADEMIA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274699]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Shanghai Inst Planted Parenthood, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Q,Wang, TL,Chen, J,et al. Human mu opioid receptor expressed in Sf9 insect cells was functionally coupled to endogenous G protein[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,1999,31(5):598-600. |
| APA | Wei, Q,Wang, TL,Chen, J,Zhou, DH,Chi, ZQ,&Shen, QX.(1999).Human mu opioid receptor expressed in Sf9 insect cells was functionally coupled to endogenous G protein.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,31(5),598-600. |
| MLA | Wei, Q,et al."Human mu opioid receptor expressed in Sf9 insect cells was functionally coupled to endogenous G protein".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 31.5(1999):598-600. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。